Repository logo
 
Publication

Chronic stress impairs the local immune response during cutaneous repair in gilthead sea bream (Sparus aurata, L.)

2017-07Journal article Open access
Simple item page
dc.contributor.authorMateus, Ana
dc.contributor.authorAnjos, Liliana
dc.contributor.authorCardoso, João CR
dc.contributor.authorPower, Deborah
dc.date.accessioned2019-11-20T15:07:31Z
dc.date.available2019-11-20T15:07:31Z
dc.date.issued2017-07
dc.description.abstractScale removal in fish triggers a damage-repair program to re-establish the lost epidermis and scale and an associated local immune response. In mammals, chronic stress is known to delay wound healing and to modulate the cutaneous stress axis, but this is unstudied in teleost fish the most successful extant vertebrates. The present study was designed to test the hypothesis that chronic stress impairs cutaneous repair in teleost fish as a consequence of suppression of the immune response. The hypothesis was tested by removing the scales and damaging the skin on one side of the body of fish previously exposed for 4 weeks to a chronic crowding stress and then evaluating cutaneous repair for 1 week. Scale removal caused the loss of the epidermis although at 3 days it was re-established. At this stage the basement membrane was significantly thicker (p = 0.038) and the hypodermis was significantly thinner (p = 0.016) in the regenerating skin of stressed fish relative to the control fish. At 3 days, stressed fish also had a significantly lower plasma osmolality (p = 0.015) than control fish indicative of reduced barrier function. Chronic stress caused a significant down-regulation of the glucocorticoid receptor (gr) in skin before damage (time 0, p = 0.005) and of star at 3 and 7 days (p < 0.05) after regeneration relative to control fish. In regenerating skin key transcripts of cutaneous repair, pcna, colival and nunp9, and the inflammatory response, tgf beta 1, mpo and crtac2, were down-regulated (p < 0.05) by chronic stress. Irrespective of chronic stress and in contrast to intact skin many hyper pigmented masses, putative melanomacrophages, infiltrated the epidermis of regenerating skin. This study reveals that chronic stress suppresses the local immune response to scale removal and impairs the expression of key transcripts of wound healing. Elements of the stress axis were identified and modulated by chronic stress during cutaneous repair in gilthead seabream skin.
dc.description.sponsorshipEuropean Regional Development Fund through COMPETE
dc.description.sponsorshipPortuguese Foundation for Science and Technology (FCT) [CCMAR/Multi/04326/2013, PTDC/MAR/ 122296/2010]
dc.description.sponsorshipFCT [UID/Multi/04326/ 2013, SFRH/BPD/79105/2011]
dc.description.sponsorshipMinistry of Science and Higher Education, Poland
dc.identifier.doi10.1016/j.molimm.2017.04.008
dc.identifier.issn0161-5890
dc.identifier.urihttp://hdl.handle.net/10400.1/13080
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationBioprospecting function of an ancient gene (CRTAC) encoding a novel protein in a marine teleost by comparative analysis
dc.subjectMessenger-Rna
dc.subjectCellular responses
dc.subjectScale regeneration
dc.subjectGrowth factors
dc.subjectTeleost fish
dc.subjectDifferential expression
dc.subjectGlucocorticoid-receptor
dc.subjectDicentrarchus-Labrax
dc.subjectBasement membrane
dc.subjectSkin regeneration
dc.subjectBarrier function
dc.subjectCutaneous wound healing
dc.subjectImmune response
dc.subjectMelanomacrophage centers
dc.subjectPeripheral stress axis
dc.titleChronic stress impairs the local immune response during cutaneous repair in gilthead sea bream (Sparus aurata, L.)
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBioprospecting function of an ancient gene (CRTAC) encoding a novel protein in a marine teleost by comparative analysis
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F122296%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F79105%2F2011/PT
oaire.citation.endPage283
oaire.citation.startPage267
oaire.citation.titleMolecular Immunology
oaire.citation.volume87
oaire.fundingStream5876
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
person.familyNameMateus
person.familyNameAnjos Guerreiro
person.familyNameCardoso
person.familyNamePower
person.givenNameAna Patrícia
person.givenNameLiliana Isabel Tomé
person.givenNameJoão
person.givenNameDeborah Mary
person.identifierAAK-3800-2020
person.identifier14332
person.identifier.ciencia-id2816-E389-626E
person.identifier.ciencia-id6D18-2D1C-A45C
person.identifier.ciencia-id8B16-F203-2AFC
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-2045-1047
person.identifier.orcid0000-0001-9474-522X
person.identifier.orcid0000-0001-7890-0170
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-4151-2013
person.identifier.scopus-author-id8575757700
person.identifier.scopus-author-id7201822956
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication74cac9a6-f4f8-438b-a60e-a2b4d3312102
relation.isAuthorOfPublication721b5dca-ad42-40f8-9731-517eb5db009e
relation.isAuthorOfPublication1b670c84-15e3-4776-8871-50f9eb0eed0d
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscovery721b5dca-ad42-40f8-9731-517eb5db009e
relation.isProjectOfPublication868b4818-3efa-4edb-9202-c464d64fd38f
relation.isProjectOfPublication8e1d6fab-06f5-4f6d-9de4-6b159c25f605
relation.isProjectOfPublicationecc1282f-5145-473b-9c12-799e39ab40f1
relation.isProjectOfPublication.latestForDiscovery8e1d6fab-06f5-4f6d-9de4-6b159c25f605

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Mateus2017_SkinStress_Mol Immunol_Accepted.pdf
Size:
1.55 MB
Format:
Adobe Portable Document Format
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)
ESS2-Artigos (em revistas ou actas indexadas)