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Carob pulp as raw material for production of the biocontrol agent P. agglomerans PBC-1

2010Journal article Restricted access
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dc.contributor.authorManso, Teresa
dc.contributor.authorNunes, Carla
dc.contributor.authorRaposo, Sara
dc.contributor.authorLima-Costa, Maria Emília
dc.date.accessioned2014-07-22T14:02:22Z
dc.date.available2014-07-22T14:02:22Z
dc.date.issued2010
dc.date.updated2014-07-22T10:11:42Z
dc.description.abstractLarge-scale production has been the major obstacle to the success of many biopesticides. The spreading of microbial biocontrol agents against postharvest disease, as a safe and environmentally friendly alternative to synthetic fungicides, is quite dependent on their industrial mass production from low-cost raw materials. Considerable interest has been shown in using agricultural waste products and by-products from food industry as nitrogen and carbon sources. In this work, carob pulp aqueous extracts were used as carbon source in the production of the biocontrol agent Pantoea agglomerans PBC-1. Optimal sugar extraction was achieved at a solid/liquid ratio of 1:10 (w/v), at 25°C, for 1 h. Batch experiments were performed in shake flasks, at different concentrations and in stirred reactors at two initial inoculums concentrations, 106 and 107 cfu ml−1. The initial sugar concentration of 5 g l−1 allowed rapid growth (0.16 h−1) and high biomass productivity (0.28 g l−1 h−1) and was chosen as the value for use in stirred reactor experiments. After 22 and 32 h of fermentation the viable population reached was 3.2 × 109 and 6.2 × 109 cfu ml−1 in the fermenter inoculated at 106 cfu ml−1 and 2.7 × 109 and 6.7 × 109 cfu ml−1 in the bioreactor inoculated at 107 cfu ml−1. A 78% reduction of the pathogen incidence was achieved with PBC-1 at 1 × 108 cfu ml−1, grown in medium with carob extracts, on artificially wounded apples stored after 7 days at 25°C against P. expansum.por
dc.identifier.citationManso, Teresa; Nunes, Carla; Raposo, Sara; Lima-Costa, Maria Emília. Carob pulp as raw material for production of the biocontrol agent P. agglomerans PBC-1, Journal of Industrial Microbiology & Biotechnology, 37, 11, 1145-1155, 2010.por
dc.identifier.doihttp://dx.doi.org/ 10.1007/s10295-010-0762-1
dc.identifier.issn1367-5435
dc.identifier.otherAUT: SRA01180;
dc.identifier.urihttp://hdl.handle.net/10400.1/4800
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringer-Verlagpor
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs10295-010-0762-1por
dc.subjectBiological controlpor
dc.subjectCarob by-productspor
dc.subjectStirred-tank reactor (STR)por
dc.subjectBatch culturespor
dc.titleCarob pulp as raw material for production of the biocontrol agent P. agglomerans PBC-1por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1155por
oaire.citation.issue11por
oaire.citation.startPage1145por
oaire.citation.titleJournal of Industrial Microbiology & Biotechnologypor
oaire.citation.volume37por
person.familyNameRaposo
person.familyNameLima Costa
person.givenNameSara
person.givenNameMaria Emília
person.identifier.ciencia-idF010-54F2-9E30
person.identifier.orcid0000-0001-5344-7349
person.identifier.orcid0000-0002-4433-5756
person.identifier.ridL-3673-2013
person.identifier.scopus-author-id7801639810
person.identifier.scopus-author-id7003633118
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication035b3010-5f47-4700-b196-402970ff2a69
relation.isAuthorOfPublication6eb9b8a6-dd51-4ace-809b-7d7de9cdfe36
relation.isAuthorOfPublication.latestForDiscovery6eb9b8a6-dd51-4ace-809b-7d7de9cdfe36

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