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Differential gene transcription, biochemical responses, and cytotoxicity assessment in Pacific oyster Crassostrea gigas exposed to ibuprofen

2015Journal article Restricted access
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dc.contributor.authorSerrano, Miguel A. S.
dc.contributor.authorGonzalez-Rey, Maria
dc.contributor.authorMattos, Jacó J.
dc.contributor.authorFlores-Nunes, Fabrício
dc.contributor.authorMello, Álvaro C. P.
dc.contributor.authorZacchi, Flávia L.
dc.contributor.authorPiazza, Clei E.
dc.contributor.authorSiebert, Marília N.
dc.contributor.authorPiazza, Rômi S.
dc.contributor.authorAlvarez-Muñoz, Diana
dc.contributor.authorRodriguez-Mozaz, Sara
dc.contributor.authorBarceló, Damià
dc.contributor.authorBebianno, Maria João
dc.contributor.authorGomes, Carlos H. A. M.
dc.contributor.authorMelo, Cláudio M. R.
dc.contributor.authorBainy, Afonso C. D.
dc.date.accessioned2020-04-22T08:09:42Z
dc.date.available2020-04-22T08:09:42Z
dc.date.issued2015
dc.description.abstractPharmaceuticals, such as anti-inflammatory nonsteroidal drugs, are frequently detected in aquatic ecosystems. Studies about the effects of these substances in nontarget organisms, such as bivalves, are relevant. The aim of this study was to evaluate the effects on antioxidant status caused by ibuprofen (IBU) in oysters Crassostrea gigas exposed for 1, 4, and 7 days at concentrations 1 and 100 μg L(-1). Levels of IBU in tissues of oysters, as well as cell viability of hemocytes, were measured. The transcription of cytochrome P450 genes (CYP2AU2, CYP356A1, CYP3071A1, CYP30C1), glutathione S-transferase isoforms (GST-ω-like and GST-π-like), cyclooxygenase-like (COX-like), fatty acid binding protein-like (FABP-like), caspase-like, heat shock protein-like (HSP70-like), catalase-like (CAT-like), and the activity of catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione S-transferase (GST) were also evaluated in the gills of oysters. The highest levels of IBU were observed in animals exposed to 100 μg L(-1). A significant upregulation of CYP2AU1, CYP356A1, CYP3071A1, GST-ω-like, GST-π-like, COX-like, and FABP-like was observed in oysters exposed to IBU under different experimental conditions. Oysters exposed to 1 μg L(-1) for 7 days showed a significantly higher transcription of CYP2AU2, CYP356A1, CYP3071A1, GST-ω-like, and GST-π-like but lower GR activity. In conclusion, C. gigas exposed to environmentally relevant concentrations of IBU (1 μg L(-1)) exhibited increased transcription of certain genes and alterations on antioxidant and auxiliary enzymes, which could, in the the long term, cause damages to exposed organisms.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s11356-014-4023-0pt_PT
dc.identifier.issn0944-1344
dc.identifier.urihttp://hdl.handle.net/10400.1/13739
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectAntioxidantspt_PT
dc.subjectCell survivalpt_PT
dc.subjectCrassostreapt_PT
dc.subjectCytotoxinspt_PT
dc.subjectDose-response relationshippt_PT
dc.subjectGillspt_PT
dc.subjectHemocytespt_PT
dc.subjectIbuprofenpt_PT
dc.subjectTranscriptionpt_PT
dc.subjectWater pollutantspt_PT
dc.subjectEcotoxicologypt_PT
dc.subjectChemicalpt_PT
dc.subjectDrugpt_PT
dc.titleDifferential gene transcription, biochemical responses, and cytotoxicity assessment in Pacific oyster Crassostrea gigas exposed to ibuprofenpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage17385pt_PT
oaire.citation.issue22pt_PT
oaire.citation.startPage17375pt_PT
oaire.citation.titleEnvironmental Science and Pollution Researchpt_PT
oaire.citation.volume22pt_PT
person.familyNameGonzalez-Rey
person.familyNameBebianno
person.givenNameMaria
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0002-9343-8460
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication182cc3b5-3e8d-4baf-9220-638978bef660
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery182cc3b5-3e8d-4baf-9220-638978bef660

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