Repository logo
 
Publication

Evaluation of MGP gene expression in colorectal cancer

2020-01Journal article Restricted
Simple item page
dc.contributor.authorCaiado, Helena
dc.contributor.authorConceição, Natércia
dc.contributor.authorTiago, Daniel
dc.contributor.authorMarreiros, Ana
dc.contributor.authorVicente, Susana
dc.contributor.authorEnriquez, Jose Luis
dc.contributor.authorVaz, Ana Margarida
dc.contributor.authorAntunes, Artur
dc.contributor.authorGuerreiro, Horacio
dc.contributor.authorCaldeira, Paulo
dc.contributor.authorLeonor Cancela, M.
dc.date.accessioned2021-06-24T11:36:01Z
dc.date.available2021-06-24T11:36:01Z
dc.date.issued2020-01
dc.description.abstractPurpose: Matrix Gla protein (MGP) is a vitamin K-dependent, gamma-carboxylated protein that was initially found to be a physiological inhibitor of ectopic calcifications affecting mainly cartilage and the vascular system. Mutations in the MGP gene were found to be responsible for a human pathology, the Keutel syndrome, characterized by abnormal calcifications in cartilage, lungs, brain and vascular system. MGP was recently implicated in tumorigenic processes such as angiogenesis and shown to be abnormally regulated in several tumors, including cervical, ovarian, urogenital and breast. This fact has triggered our interest in analyzing the expression of MGP and of its regulator, the transcription factor runt related transcription factor 2 (RUNX2), in colorectal cancer (CRC). Methods: MGP and RUNX2 expression were analyzed in cancer and non-tumor biopsies samples from 33 CRC patients and 9 healthy controls by RT-qPCR. Consequently, statistical analyses were performed to evaluate the clinical-pathological significance of MGP and RUNX2 in CRC. MGP protein was also detected by immunohistochemical analysis. Results: Showed an overall overexpression of MGP in the tumor mucosa of patients at mRNA level when compared to adjacent normal mucosa and healthy control tissues. In addition, analysis of the expression of RUNX2 mRNA demonstrated an overexpression in CRC tissue samples and a positive correlation with MGP expression (Pearson correlation coefficient 0.636; p <= 0.01) in tumor mucosa. However correlations between MGP gene expression and clinical-pathological characteristics, such as gender, age and pathology classification did not provide relevant information that may shed light towards the differences of MGP expression observed between normal and malignant tissue. Conclusions: We were able to associate the high levels of MGP mRNA expression with a worse prognosis and survival rate lower than five years. These results contributed to improve our understanding of the molecular mechanism underlying MGP deregulation in cancer.
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1016/j.gene.2019.144120
dc.identifier.issn0378-1119
dc.identifier.urihttp://hdl.handle.net/10400.1/16605
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationCentre of Marine Sciences
dc.relationFunctional analysis of OPTN and SQSTM1 mutations in Paget´s disease of bone
dc.relationImpact of microRNAs in skeleton formation and regeneration
dc.relationMolecular basis for the epigenetic regulation of MGP in cancer
dc.subjectColorectal Cancer (CRC)
dc.subjectMatrix gla protein
dc.subjectGene expression
dc.subjectTranscription factors
dc.subjectRUNX2
dc.subject.otherGenetics & Heredity
dc.titleEvaluation of MGP gene expression in colorectal cancer
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Marine Sciences
oaire.awardTitleFunctional analysis of OPTN and SQSTM1 mutations in Paget´s disease of bone
oaire.awardTitleImpact of microRNAs in skeleton formation and regeneration
oaire.awardTitleMolecular basis for the epigenetic regulation of MGP in cancer
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04326%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F111898%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F111289%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/PD%2FBD%2F128341%2F2017/PT
oaire.citation.startPage144120
oaire.citation.titleGene
oaire.citation.volume723
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
person.familyNameCaiado
person.familyNameConceição
person.familyNameTiago
person.familyNameMarreiros
person.familyNameVicente
person.familyNameGuerreiro
person.familyNameCaldeira
person.familyNameCancela
person.givenNameHelena
person.givenNameNatércia
person.givenNameDaniel
person.givenNameAna
person.givenNameSusana Sofia
person.givenNameHoracio
person.givenNamePaulo
person.givenNameM. Leonor
person.identifier.ciencia-id7D19-F0E4-4166
person.identifier.ciencia-id7C11-760D-F425
person.identifier.ciencia-id9A12-9450-7051
person.identifier.ciencia-id181E-54F9-E4E1
person.identifier.ciencia-id1112-F0B2-19A3
person.identifier.orcid0000-0002-2754-8266
person.identifier.orcid0000-0002-5057-0912
person.identifier.orcid0000-0001-8418-6292
person.identifier.orcid0000-0001-9410-4772
person.identifier.orcid0000-0002-1387-5813
person.identifier.orcid0000-0002-2404-6295
person.identifier.orcid0000-0003-3114-6662
person.identifier.scopus-author-id14422711000
person.identifier.scopus-author-id57194785077
person.identifier.scopus-author-id57188667152
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublicationcac8ae37-2d50-4112-a9ab-da372b75209f
relation.isAuthorOfPublication62064ac0-1f34-407d-96b7-b7074325a84e
relation.isAuthorOfPublication10cda3a0-7b9c-4d16-bd2a-a06df1d56d94
relation.isAuthorOfPublicationc0a8e5da-26ae-42a8-ab04-fa4df4356375
relation.isAuthorOfPublication50d5fbb5-9040-4a9e-bf90-af6acb08a6b7
relation.isAuthorOfPublication76efcc1f-bb58-4a5b-b4f5-9f206622df4f
relation.isAuthorOfPublication3def3720-8982-4c46-801d-af640d508b59
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublication.latestForDiscovery50d5fbb5-9040-4a9e-bf90-af6acb08a6b7
relation.isProjectOfPublicationf38dbd9d-3734-4f3c-8d74-dff8dcaa8674
relation.isProjectOfPublication6ce8901f-f4c0-405e-b124-44ab829c618e
relation.isProjectOfPublication8a4804db-2000-45c8-91f2-6866af688558
relation.isProjectOfPublication5b57d7d2-eb8d-475d-be8f-ab635c8ce0f6
relation.isProjectOfPublication.latestForDiscoveryf38dbd9d-3734-4f3c-8d74-dff8dcaa8674

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Evaluation of MGP gene expression in colorectal cancer.pdf
Size:
2.54 MB
Format:
Adobe Portable Document Format
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)
FCB2-Artigos (em revistas ou actas indexadas)