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Genistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrier

2019Journal article Restricted access
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dc.contributor.authorPinto, Patricia IS
dc.contributor.authorAndrade, André
dc.contributor.authorMoreira, Catarina
dc.contributor.authorZapater, Cinta
dc.contributor.authorThorne, Michael A.S.
dc.contributor.authorSantos, Soraia
dc.contributor.authorEstêvão, M. Dulce
dc.contributor.authorGomez, Ana
dc.contributor.authorCanario, Adelino
dc.contributor.authorPower, Deborah
dc.date.accessioned2020-03-23T10:04:35Z
dc.date.available2020-12-01T01:30:14Z
dc.date.issued2019
dc.description.abstractTeleost fish scales play important roles in animal protection and homeostasis. They can be targeted by endogenous estrogens and by environmental estrogenic endocrine disruptors. The phytoestrogen genistein is ubiquitous in the environment and in aquaculture feeds and is a disruptor of estrogenic processes in vertebrates. To test genistein disrupting actions in teleost fish we used a minimally invasive approach by analysing scales plucked from the skin of sea bass (Dicentrarchus labrax). Genistein transactivated all three fish nuclear estrogen receptors and was most potent with the Esr2, had the highest efficacy with Esr1, but reached, in all cases, transactivation levels lower than those of estradiol. RNA-seq revealed 254 responsive genes in the sea bass scales transcriptome with an FDR < 0.05 and more than 2-fold change in expression, 1 or 5 days after acute exposure to estradiol or to genistein. 65 genes were specifically responsive to estradiol and 106 by genistein while 83 genes were responsive to both compounds. Estradiol specifically regulated genes of protein/matrix turnover and genistein affected sterol biosynthesis and regeneration, while innate immune responses were affected by both compounds. This comprehensive study revealed the impact on the fish scale transcriptome of estradiol and genistein, providing a solid background to further develop fish scales as a practical screening tool for endocrine disrupting chemicals in teleosts.pt_PT
dc.description.sponsorshipAGL2015-67477-C2-1-R
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.jsbmb.2019.105448pt_PT
dc.identifier.issn0960-0760
dc.identifier.urihttp://hdl.handle.net/10400.1/13636
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPergamon-Elsevierpt_PT
dc.relationE2SCALE: Scaling-down estrogenic responses in fish
dc.relationCentre of Marine Sciences
dc.subjectAnimal scalespt_PT
dc.subjectAnimalspt_PT
dc.subjectBasspt_PT
dc.subjectEndocrine disruptorspt_PT
dc.subjectEstradiolpt_PT
dc.subjectGenisteinpt_PT
dc.subjectHEK293 cellspt_PT
dc.subjectHumanspt_PT
dc.subjectReceptorspt_PT
dc.subjectSkinpt_PT
dc.subjectTranscriptomept_PT
dc.titleGenistein and estradiol have common and specific impacts on the sea bass (Dicentrarchus labrax) skin-scale barrierpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleE2SCALE: Scaling-down estrogenic responses in fish
oaire.awardTitleCentre of Marine Sciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAAG-GLO%2F4003%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F84033%2F2012/PT
oaire.citation.startPage105448pt_PT
oaire.citation.titleThe Journal of Steroid Biochemistry and Molecular Biologypt_PT
oaire.citation.volume195pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamSFRH
person.familyNamePinto
person.familyNameAlçada Baptista Rebelo de Andrade
person.familyNameSantos
person.familyNameEstêvão
person.familyNameCanario
person.familyNamePower
person.givenNamePatricia IS
person.givenNameAndré
person.givenNameSoraia
person.givenNameMaria Dulce da Mota Antunes de Oliveira
person.givenNameAdelino
person.givenNameDeborah Mary
person.identifier643457
person.identifierI-4695-2014
person.identifier143624
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.ciencia-idF513-56ED-7088
person.identifier.ciencia-idEF18-F0AF-D388
person.identifier.ciencia-id881B-53E8-0DB1
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-7854-3898
person.identifier.orcid0000-0002-5900-8782
person.identifier.orcid0000-0001-5006-2021
person.identifier.orcid0000-0002-7151-8363
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-3817-2013
person.identifier.ridC-1328-2018
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id10240774300
person.identifier.scopus-author-id8381590400
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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