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Cryopreservation of pollen of carob tree

2006Journal article Restricted access
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dc.contributor.authorCustódio, Luísa
dc.contributor.authorRomano, Anabela
dc.contributor.authorFernandes, N.
dc.contributor.authorCarneiro, M. F.
dc.date.accessioned2015-06-18T10:12:38Z
dc.date.available2015-06-18T10:12:38Z
dc.date.issued2006
dc.description.abstractIn this work, anthers isolated from male flowers of carob tree (Ceratonia siliqua L.) at developmental phase II were cryopreserved by vitrification, using a fast freezing method. Different types of carbohydrates at different concentrations, namely sorbitol (0.5 M, 1 M, and 2 M), mannitol (0.5 M and 1 M), sucrose (0.5 M, 1 M, and 2 M), and glucose (0.5 M, I M, and 2 M), namely sorbitol, mannitol, sucrose and glucose, were compared with respect to their capacity of inducing freeze tolerance in pollen of carob tree. Carbohydrates were applied as a pre-treatment to anthers before cryostorage. It was also assessed if the presence of the cryoprotectant during the storage period was beneficial for pollen viability. The viability of cryopreserved pollen was evaluated after 5 and 8 months of storage. The application of the cryoprotectants generally increased pollen viability as compared with the control. The best results were obtained after 5 months storage, in the presence of the cryoprotector, with pollen pre-treated with sucrose 0.5 M. The viability of pollen decreased with increasing the duration of storage period.
dc.identifier.isbn978-90-6605-719-7
dc.identifier.issn0567-7572
dc.identifier.otherAUT: ARO01015;
dc.identifier.urihttp://hdl.handle.net/10400.1/6434
dc.language.isoeng
dc.peerreviewedyes
dc.publisherInternational Society for Horticultural Science
dc.relation.isbasedonP-004-QMP
dc.relation.ispartofActa Horticulturae
dc.subjectAndrogenesis
dc.subjectCeratonia siliqua L.
dc.subjectGermplasm
dc.subjectHaploids
dc.subjectMicrospore
dc.titleCryopreservation of pollen of carob tree
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F1274%2F2000/PT
oaire.citation.conferencePlaceDebrecen, Hungary
oaire.citation.endPage867
oaire.citation.startPage863
oaire.citation.titleProceedings of the Vth International Symposium on in Vitro Culture and Horticulture Breeding, Vols 1 and 2
oaire.citation.title5th International Symposium on In Vitro Culture and Horticultural Breeding
oaire.citation.volume725
oaire.fundingStreamSFRH
person.familyNameCustódio
person.familyNameRomano
person.givenNameLuísa
person.givenNameAnabela
person.identifier.ciencia-id791B-C560-AEA2
person.identifier.ciencia-id8A18-7E9A-3B0E
person.identifier.orcid0000-0003-4338-7703
person.identifier.orcid0000-0002-7204-7428
person.identifier.ridM-6101-2013
person.identifier.scopus-author-id15831018900
person.identifier.scopus-author-id56249997600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublicationf9cfed0f-6b67-413e-988c-ac7397183471
relation.isAuthorOfPublicatione3343b60-17d3-4f5e-a2bf-06f26da69206
relation.isAuthorOfPublication.latestForDiscoveryf9cfed0f-6b67-413e-988c-ac7397183471
relation.isProjectOfPublicationee748308-8542-4aca-b72d-52c63223dbe7
relation.isProjectOfPublication.latestForDiscoveryee748308-8542-4aca-b72d-52c63223dbe7

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