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Brain aromatase from pejerrey fish (Odontesthes bonariensis): cDNA cloning, tissue expression, and immunohistochemical localization

2005Journal article Restricted
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dc.contributor.authorStrobl-Mazzulla, Pablo H.
dc.contributor.authorMoncaut, Natalia P.
dc.contributor.authorLópez, Gabriela C.
dc.contributor.authorMiranda, Leandro A.
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorSomoza, G. M.
dc.date.accessioned2014-05-12T08:39:53Z
dc.date.available2014-05-12T08:39:53Z
dc.date.issued2005
dc.date.updated2014-05-09T08:51:23Z
dc.description.abstractThe brain-type aromatase (CYP19A2) cDNA from pejerrey Odontesthes bonariensis was characterized. Its sequence differs from the ovarian-derived aromatase (CYP19A1) previously reported for the same species. The cDNA is 2305 bp in length and the deduced protein comprises 501 amino-acids. The percentage of identity was higher when compared to other brain-derived aromatase proteins (85–63%) and lower with ovarian-derived aromatases (64–57%). Pejerrey aromatases share 61% of identity. The tissue expression analysis showed that CYP19A2 was expressed in the kidney, brain, and pituitary gland of both sexes and also in the ovary, but not in the eye, spleen, liver, gill, and testis. Semi-quantitative RT-PCR analysis of different brain areas revealed that CYP19A2 was expressed significantly higher in anterior male brain areas than in the corresponding female areas, and also when compared to posterior brain areas from both sexes. An immunological analysis using a polyclonal anti-teleost aromatase showed immunoreactive aromatase cells bordering the telencephalic ventricle and a strong signal in the ependymal cells of the preoptic area and the hypothalamus. In the optic tectum immunoreactive aromatase cells were labeled in the ventral wall and in the ependymal layer of the third and fourth ventricle with lateral projections. In the pituitary gland immunoreactive aromatase cells could be found in the rostral and proximal pars distalis. In this gland, aromatase fibers were also detected in different areas; many of them concentrated around blood vessels.por
dc.identifier.citationStrobl-Mazzulla, Pablo H.; Moncaut, Natalia P.; López, Gabriela C.; Miranda, Leandro A.; Canario, Adelino V.M.; Somoza, Gustavo M.Brain aromatase from pejerrey fish (Odontesthes bonariensis): cDNA cloning, tissue expression, and immunohistochemical localization, General and Comparative Endocrinology, 143, 1, 21-32, 2005.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.ygcen.2005.02.026
dc.identifier.issn0016-6480
dc.identifier.otherAUT: ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3848
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0016648005000936por
dc.subjectBrainpor
dc.subjectAromatasepor
dc.subjectSex steroidspor
dc.subjectPejerreypor
dc.titleBrain aromatase from pejerrey fish (Odontesthes bonariensis): cDNA cloning, tissue expression, and immunohistochemical localizationpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage32por
oaire.citation.issue1por
oaire.citation.startPage21por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume143por
person.familyNameCanario
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521

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