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Carob trees (Ceratonia siliqua L.) regenerated in vitro can acclimatize successfully to match the field performance of seed-derived plants

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dc.contributor.authorOsório, Maria Leonor
dc.contributor.authorOsório, Júlio
dc.contributor.authorGonçalves, Sandra
dc.contributor.authorDavid, Maria Manuela
dc.contributor.authorRomano, Anabela
dc.contributor.authorCorreia, Maria João
dc.date.accessioned2014-06-30T12:53:02Z
dc.date.available2014-06-30T12:53:02Z
dc.date.issued2012
dc.description.abstractThe use of in vitro regenerated plants in forestry and orchard depends ultimately on the development of efficient transplantation protocols, ensuring high survival rates and successful establishment under field conditions. We tested the performance of micropropagated carob trees (Ceratonia siliqua L.) throughout the acclimatization process in terms of survival, growth and physiological traits, including field comparisons with seed-derived and mother plants. The field trial was 100 %successful, i.e. we found no major differences between micropropagated, seed-derived and mother plants in terms of growth rate, height, number of leaves, photosynthetic efficiency, chlorophyll fluorescence, chlorophyll content and soluble protein content, although these parameters changed significantly during acclimatization. Stomatal conductance (gs) was reduced by fourfold when plants were transferred from in vitro culture to the growth chamber, thus preventing uncontrolled wilting. The photosynthetic rate (PN) was relatively low in vitro, in the growth chamber and the greenhouse, but increased to match seed-derived and mother plants in the field. The chlorophyll a/b ratio in leaves from in vitro and growth chamber plants was typical of shade plants (2.1) but became more characteristic of sun plants in the subsequent acclimatization stages (3.1–3.5). The maximum efficiency of photosystem II (Fv/Fm) remained mostly constant at ~0.80 throughout acclimatization, as is typical for healthy, non-stressed plants. We conclude that our micropropagation and acclimatization protocols provide a suitable alternative to traditional mass propagation techniques for the carob tree.por
dc.description.sponsorshipM.L. Osório and S. Gonçalves acknowledge a grant from the Portuguese Science and Technology Foundation (FCT, Grant SFRH/BPD/35410/2007 and SFRH/BPD/31534/2006)por
dc.identifier.citationOsório ML, Osório J, Gonçalves S, David MM, Correia MJ, Romano A 2012. Carob trees (Ceratonia siliqua L.) regenerated in vitro can acclimatize successfully to match the field performance of seed-derived plants. Trees - Structure and Function 26:1837–1846por
dc.identifier.doihttp://dx.doi.org/10.1007/s00468-012-0753-0
dc.identifier.otherAUT: JOS01477; MDA00369; JCO00381; ARO01015
dc.identifier.urihttp://hdl.handle.net/10400.1/4586
dc.language.isoporpor
dc.peerreviewedyespor
dc.publisherSpringer Verlagpor
dc.relation.publisherversionhttp://link.springer.com/article/10.1007/s00468-012-0753-0por
dc.subjectAcclimatizationpor
dc.subjectChlorophyll fluorescencepor
dc.subjectMicropropagationpor
dc.subjectPhotosynthesispor
dc.subjectStomatal conductancepor
dc.titleCarob trees (Ceratonia siliqua L.) regenerated in vitro can acclimatize successfully to match the field performance of seed-derived plantspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1846por
oaire.citation.issue6por
oaire.citation.startPage1837por
oaire.citation.titleTrees - Structure and Functionpor
oaire.citation.volume26por
person.familyNameOsório
person.familyNameOsório
person.familyNameGonçalves
person.familyNameDavid
person.familyNameRomano
person.givenNameMaria Leonor
person.givenNameJúlio
person.givenNameSandra
person.givenNameMaria Manuela
person.givenNameAnabela
person.identifier.ciencia-id9F19-E3AE-2C59
person.identifier.ciencia-idE31B-16E0-7562
person.identifier.ciencia-id8A18-7E9A-3B0E
person.identifier.orcid0000-0002-7713-6497
person.identifier.orcid0000-0002-4054-4091
person.identifier.orcid0000-0002-3038-4434
person.identifier.orcid0000-0001-6251-7222
person.identifier.orcid0000-0002-7204-7428
person.identifier.ridM-6988-2017
person.identifier.ridN-8024-2013
person.identifier.scopus-author-id7006394339
person.identifier.scopus-author-id9938818600
person.identifier.scopus-author-id8583003200
person.identifier.scopus-author-id7402103697
person.identifier.scopus-author-id56249997600
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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