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Genetic variants of Grapevine leafroll-associated virus 2 infecting Portuguese grapevine cultivars

2016Journal article Open access
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dc.contributor.authorFonseca, Filomena
dc.contributor.authorEsteves, Filipa
dc.contributor.authorTeixeira Santos, Margarida
dc.contributor.authorBrazão, João
dc.contributor.authorEiras-Dias, José Eduardo
dc.date.accessioned2017-04-07T15:57:35Z
dc.date.available2017-04-07T15:57:35Z
dc.date.issued2016
dc.description.abstractGenetic variability of 19 isolates of Grapevine leafroll-associated virus 2 (GLRaV-2) from Portuguese grapevine cultivars was characterized by sequencing the entire capsid protein (CP) gene of the virus. Global phylogenetic analysis of the CP gene, which included nucleotide sequences obtained in this study and complete homologous sequences from GenBank, showed segregation of GLRaV-2 variants from Portuguese isolates into three major phylogroups (PN, 93/955 and H4). The novelty of these phylogenetic results is the evidence of well-supported subdivision within H4 as well as within PN, with subgroup PN3 composed exclusively of variants from a Portuguese isolate. These findings and the genetic analysis of global phylogroups indicate demographic expansion, mainly within PN and 93/955. Because the existence of a mixture of variants from different phylogroups was detected in some of the isolates, a typification assay based on reverse transcription reaction followed by polymerase chain reaction and restriction fragment length polymorphism analysis, was developed to complement molecular detection assay of the virus. This protocol discriminates variants from the phylogroups identified in this study, and is appropriate for routine testing for GLRaV-2.
dc.description.sponsorshipPTDC/AGR-ALI/109869/2009
dc.identifier.doi10.14601/Phytopathol_Mediterr-16378
dc.identifier.issn0031-9465
dc.identifier.urihttp://hdl.handle.net/10400.1/9753
dc.language.isoeng
dc.peerreviewedyes
dc.relationMolecular re-assessment of taxonomic units within Ampelovirus and its implications under the new grapevine certification scheme
dc.relation.isbasedonWOS:000376104400006
dc.titleGenetic variants of Grapevine leafroll-associated virus 2 infecting Portuguese grapevine cultivars
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMolecular re-assessment of taxonomic units within Ampelovirus and its implications under the new grapevine certification scheme
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-AAM%2F65094%2F2006/PT
oaire.citation.endPage88
oaire.citation.issue1
oaire.citation.startPage73
oaire.citation.titlePhytopathologia Mediterranea
oaire.citation.volume55
oaire.fundingStream3599-PPCDT
person.familyNameFonseca
person.givenNameFilomena
person.identifier.orcid0000-0003-3824-0459
person.identifier.scopus-author-id7004434992
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication8eb52d8c-f5c0-4ac5-9dbb-a985321045a3
relation.isAuthorOfPublication.latestForDiscovery8eb52d8c-f5c0-4ac5-9dbb-a985321045a3
relation.isProjectOfPublication4845c2f6-2fbe-4894-ac4e-887b7ac1bca2
relation.isProjectOfPublication.latestForDiscovery4845c2f6-2fbe-4894-ac4e-887b7ac1bca2

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