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Establishment of an in vitro propagation protocol for Thymus lotocephalus, a rare aromatic species of the Algarve (Portugal)

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dc.contributor.authorCoelho, Natacha
dc.contributor.authorGonçalves, Sandra
dc.contributor.authorGonzález-Benito, María Elena
dc.contributor.authorRomano, Anabela
dc.date.accessioned2013-03-15T11:45:50Z
dc.date.available2013-03-15T11:45:50Z
dc.date.issued2012
dc.description.abstractThe aim of this work was to develop an in vitro propagation protocol for the endangered species Thymus lotocephalus using seedlings as explants. Several macronutrient concentrations of Murashige and Skoog medium (MS), cytokinin types and concentrations, and cytokinin/auxin combinations were tested to assess the shoots’ proliferation capacity. Although the best proliferation results were obtained with 6-benzyladenine, high percentages of hyperhidric shoots were observed. Because high proliferation of healthy shoots was observed in MS medium that was free of plant growth regulators, this medium was chosen for proliferation studies. The best rooting results were achieved in MS medium without auxins (92.00 ± 6.11%, 6.54 ± 0.52 and 1.60 ± 0.10 cm regarding rooting frequency, number of roots per shoot and longest roots, respectively) or supplemented with 0.5 mg l-1 indole-3-acetic acid (98.00 ± 2.11%, 11.14 ± 0.75 and 2.40 ± 0.24 cm, respectively). Plantlets were successfully acclimatised to ex vitro conditions with a survival rate of 93.33%. With the development of this micropropagation protocol, an important contribution has been made to the conservation of the endangered species T. lotocephalus.por
dc.description.sponsorshipN Coelho and S Gonçalves acknowledge a grant from the Portuguese Science and Technology Foundation (FCT, grants SFRH/BD/63501/2009 and SFRH/BPD/31534/2006, respectively).por
dc.identifier.citationCoelho N., Gonçalves S., González-Benito M. E., Romano A. 2012. Establishment of an in vitro propagation protocol for Thymus lotocephalus, a rare aromatic species of the Algarve (Portugal), Plant Growth Regulation 66: 69–74por
dc.identifier.issn0167-6903
dc.identifier.otherAUT: ARO01015;
dc.identifier.urihttp://hdl.handle.net/10400.1/2585
dc.language.isoengpor
dc.peerreviewedyespor
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs10725-011-9630-xpor
dc.subjectAcclimatisationpor
dc.subjectEndangered speciespor
dc.subjectConservationpor
dc.subjectHyperhydricitypor
dc.subjectLamiaceaepor
dc.subjectRootingpor
dc.subjectSeed germinationpor
dc.titleEstablishment of an in vitro propagation protocol for Thymus lotocephalus, a rare aromatic species of the Algarve (Portugal)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage74por
oaire.citation.issue1por
oaire.citation.startPage69por
oaire.citation.titlePlant Growth Regulationpor
oaire.citation.volume66por
person.familyNameCoelho
person.familyNameGonçalves
person.familyNameRomano
person.givenNameNatacha
person.givenNameSandra
person.givenNameAnabela
person.identifier.ciencia-idC518-BDB3-E127
person.identifier.ciencia-id9F19-E3AE-2C59
person.identifier.ciencia-id8A18-7E9A-3B0E
person.identifier.orcid0000-0003-4265-5622
person.identifier.orcid0000-0002-3038-4434
person.identifier.orcid0000-0002-7204-7428
person.identifier.ridM-6988-2017
person.identifier.scopus-author-id8583003200
person.identifier.scopus-author-id56249997600
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication43b2d7ec-049a-412b-ad22-c9db64cc572c
relation.isAuthorOfPublicationa8b335b2-45cc-4578-89b0-153273014280
relation.isAuthorOfPublicatione3343b60-17d3-4f5e-a2bf-06f26da69206
relation.isAuthorOfPublication.latestForDiscovery43b2d7ec-049a-412b-ad22-c9db64cc572c

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