Cloning and expression of an elongation factor-1α in sea bream ( Sparus aurata ) larvae and adult tissue

Nowell, M. A.; Power, Deborah; Guerreiro, P. M.; Llewellyn, Lynda; Ramsurn, Vimi P.; Wigham, Trevor; Sweeney, Glen E.

http://hdl.handle.net/10400.1/5386

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Abstract(s)

A clone encoding the polypeptide elongation factor EF-1a was isolated from a complementary DNA library prepared from sea bream (Spartus aurata) larvae 1 to 10 days after hatching. The deduced amino acid sequence is between 82% and 95% similar to EF-1a in other animal species. EF-1a messenger RNA is present at low abundance in sea bream embryos prior to gastrulation, but at around 15 hours postfertilization, there is a 10-fold increase in transcript levels. This increase presumably reflects midblastula transition in this species. In adult sea bream, EF-1a appeared to have a relatively uniform distribution across all the tissues analyzed.

Keywords

EF-1a cDNA Expression Sea beam (sparus aurata) Teleost Larvae Adult tissue

URI

http://hdl.handle.net/10400.1/5386

Citation

Mari A. Nowell, Deborah M. Power, Pedro M. Guerreiro, Lynda Llewellyn, Vimi Ramsurn, Trevor Wigham and Glen E. Sweeney. Cloning and Expression of an Elongation Factor-1a in Sea Bream (Sparus aurata) Larvae and Adult Tissue. Mar. Biotechnol. 2, 173–179, 2000