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eDNA metabarcoding for diet analyses of green sea turtles (Chelonia mydas)

2021Journal article Open access
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dc.contributor.authorDíaz-Abad, Lucía
dc.contributor.authorBacco-Mannina, Natassia
dc.contributor.authorMadeira, Fernando Miguel
dc.contributor.authorNeiva, J.
dc.contributor.authorAires, Tania
dc.contributor.authorSerrao, Ester
dc.contributor.authorRegalla, Aissa
dc.contributor.authorPatrício, Ana R.
dc.contributor.authorRodrigues Frade, Pedro
dc.date.accessioned2022-01-18T14:31:53Z
dc.date.available2022-01-18T14:31:53Z
dc.date.issued2021
dc.description.abstractUnderstanding sea turtle diets can help conservation planning, but their trophic ecology is complex due to life history characteristics such as ontogenetic shifts and large foraging ranges. Studying sea turtle diet is challenging, particularly where ecological foraging observations are not possible. Here, we test a new minimally invasive method for the identifcation of diet items in sea turtles. We fngerprinted diet content using DNA from esophageal and cloacal swab samples by metabarcoding the 18S rRNA gene. This approach was tested on samples collected from green turtles (Chelonia mydas) from a juvenile foraging aggregation in the Bijagós archipelago in Guinea-Bissau. Esophagus samples (n=6) exhibited a higher dietary richness (11±5 amplicon sequence variants (ASVs) per sample; average±SD) than cloacal ones (n=5; 8±2 ASVs). Overall, the diet was dominated by red macroalgae (Rhodophyta; 48.2±16.3% of all ASVs), with the main food item in the esophagus and cloaca being a red alga belonging to the Rhodymeniophycidae subclass (35.1±27.2%), followed by diatoms (Bacillariophyceae; 7.5±7.3%), which were presumably consumed incidentally. Seagrass and some invertebrates were also present. Feeding on red algae was corroborated by feld observations and barcoding of food items available in the benthic habitat, validating the approach for identifying diet content. We conclude that identifcation of food items using metabarcoding of esophageal swabs is useful for a better understanding of the relationships between the feeding behavior of sea turtles and their environment.pt_PT
dc.description.sponsorshipNORTE-01-0145-FEDER-000046
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00227-021-04002-xpt_PT
dc.identifier.eissn1432-1793
dc.identifier.urihttp://hdl.handle.net/10400.1/17492
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.relationExpanding potential in TROPIcal BIOdiversity and ecosystem research towards sustainable life on land
dc.relationThe gastric cavity of corals as bioreactor fo ecosystem-scale microbial processes
dc.relationHotspots de biodiversidade genética em florestas marinhas
dc.relationMarine macrophytes holobionts: Acclimation and adaptation mechanisms across oceans in a global change scenario
dc.relationNot Available
dc.relationMarine and Environmental Sciences Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDNA barcodingpt_PT
dc.subjectGuinea-Bissaupt_PT
dc.subjectMacrophytespt_PT
dc.subjectGreen turtlept_PT
dc.subjectFeeding ecologypt_PT
dc.subjectMarine biodiversitypt_PT
dc.titleeDNA metabarcoding for diet analyses of green sea turtles (Chelonia mydas)pt_PT
dc.title.alternativeMetabarificação eDNA para análises dietéticas de tartarugas marinhas verdes (Chelonia mydas)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleExpanding potential in TROPIcal BIOdiversity and ecosystem research towards sustainable life on land
oaire.awardTitleThe gastric cavity of corals as bioreactor fo ecosystem-scale microbial processes
oaire.awardTitleHotspots de biodiversidade genética em florestas marinhas
oaire.awardTitleMarine macrophytes holobionts: Acclimation and adaptation mechanisms across oceans in a global change scenario
oaire.awardTitleNot Available
oaire.awardTitleMarine and Environmental Sciences Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/854248/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBPD%2F110285%2F2015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBSAB%2F150485%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBPD%2F116774%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL 57%2F2016%2FCP1361%2FCT0010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04292%2F2020/PT
oaire.citation.issue1pt_PT
oaire.citation.titleMarine Biologypt_PT
oaire.citation.volume169pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamH2020
oaire.fundingStreamOE
oaire.fundingStreamOE
oaire.fundingStreamOE
oaire.fundingStreamDL 57/2016
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMiranda Neiva
person.familyNameAires
person.familyNameSerrao
person.familyNameRodrigues Frade
person.givenNameJoão
person.givenNameTania
person.givenNameEster A.
person.givenNamePedro
person.identifierM-3818-2013
person.identifier282868
person.identifierC-6686-2012
person.identifier.ciencia-id191B-5464-41F5
person.identifier.ciencia-id4318-9E2C-32B9
person.identifier.ciencia-id5B13-B26E-B1EC
person.identifier.orcid0000-0002-5927-4570
person.identifier.orcid0000-0002-1964-6819
person.identifier.orcid0000-0003-1316-658X
person.identifier.orcid0000-0002-4010-255X
person.identifier.ridM-8306-2013
person.identifier.scopus-author-id54895771800
person.identifier.scopus-author-id7004093604
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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