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In vitro plantlet production of the endangered Pinguicula vulgaris

dc.contributor.authorGrevenstuk, Tomás
dc.contributor.authorRomano, Anabela
dc.date.accessioned2015-06-18T10:12:38Z
dc.date.available2015-06-18T10:12:38Z
dc.date.issued2012
dc.description.abstractThis study describes the development of a micropropagation protocol for Pinguicula vulgaris using cultures initiated from in vitro produced seedlings. P. vulgaris is a carnivorous plant with a northern, disjunctly circumpolar distribution and specific habitat requirements, and is hence becoming increasingly rare. Shoot proliferation was significantly influenced by Murashige and Skoog (MS) macronutrient concentration, showing higher proliferation rates in 1/4MS, but was not affected by the addition of 0.1 mg/L 6-benzyladenine (BA) or zeatin (Zea). The best medium for propagating P. vulgaris was plant growth regulator (PGR) free A1/4MS. An average of 7.62 new shoots per initial explant could be obtained after 8 weeks of culture, of which over 79% produced roots during proliferation. Moreover, rooting percentages of 100% were obtained for the initial explants in all the tested media, including media without PGRs. The plantlets were successfully acclimatized to ex vitro conditions, exhibiting normal development.
dc.identifier.doihttps://dx.doi.org/10.2478/s11535-011-0103-z
dc.identifier.issn1895-104X
dc.identifier.otherAUT: ARO01015;
dc.identifier.urihttp://hdl.handle.net/10400.1/6433
dc.language.isoeng
dc.peerreviewedyes
dc.publisherVersita
dc.relation.isbasedonP-002-DQM
dc.subjectConservation
dc.subjectIn vitro regeneration
dc.subjectPlant growth regulators
dc.subjectCarnivorous plant
dc.titleIn vitro plantlet production of the endangered Pinguicula vulgaris
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage53
oaire.citation.issue1
oaire.citation.startPage48
oaire.citation.titleCentral European Journal of Biology
oaire.citation.volume7
person.familyNameGrevenstuk
person.familyNameRomano
person.givenNameTomás
person.givenNameAnabela
person.identifier.ciencia-idB31A-EC35-5A31
person.identifier.ciencia-id8A18-7E9A-3B0E
person.identifier.orcid0000-0003-0779-3018
person.identifier.orcid0000-0002-7204-7428
person.identifier.scopus-author-id24329026400
person.identifier.scopus-author-id56249997600
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication4ae6491f-f21f-4001-8987-dec1ad73ca5c
relation.isAuthorOfPublicatione3343b60-17d3-4f5e-a2bf-06f26da69206
relation.isAuthorOfPublication.latestForDiscovery4ae6491f-f21f-4001-8987-dec1ad73ca5c

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