Proliferative and mineralogenic effects of insulin, IGF-1, and vanadate in fish osteoblast-like cells

Tiago, Daniel; Cancela, Leonor; Laizé, Vincent

http://hdl.handle.net/10400.1/4280

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Authors

Tiago, Daniel

Cancela, Leonor

Laizé, Vincent

Abstract(s)

Fish have recently been recognized as a suitable model and a promising alternative to mammalian systems to study skeletogenesis. In this regard, several fish bone-derived cell lines have been developed and are being used to investigate mechanisms associated with insulin-like action of vanadium on extracellular matrix (ECM) mineralization. Although proliferative and mineralogenic effects of vanadate, insulin-like growth factor 1 (IGF-1), and insulin have recently been evaluated in a fish prechondrocyte cell line, no data are available in fish bone-forming cells, the osteoblasts. Using fish preosteoblast cells, we showed that IGF-1, but not insulin or vanadate, stimulated cell proliferation through the mitogen-activated protein kinase (MAPK) pathway, while both IGF-1 and vanadate inhibited cell differentiation/ECM mineralization through the same mechanism. Our data also indicated that the phosphatidyl inositol-3 kinase (PI-3K) pathway stimulates differentiation/ECM mineralization in osteoblasts and could represent a way to balance MAPK pathway action. The comparison of these new data obtained in fish with those available in mammals clearly evidenced a conservation of regulatory mechanisms among vertebrate bone-derived systems, although different players are involved.

Keywords

Vanadium Insulin-like activity Intracellular signaling pathway In vitro mineralization Bony fish osteoblast

URI

http://hdl.handle.net/10400.1/4280

Citation

Tiago, D.M.; Cancela, M.L.; Laizé, V.Proliferative and mineralogenic effects of insulin, IGF-1, and vanadate in fish osteoblast-like cells, Journal of Bone and Mineral Metabolism, 29, 3, 377-382, 2011.