Repository logo
 
Publication

Proliferative and mineralogenic effects of insulin, IGF-1, and vanadate in fish osteoblast-like cells

2011-05Journal article Restricted
Simple item page
dc.contributor.authorTiago, Daniel
dc.contributor.authorCancela, Leonor
dc.contributor.authorLaizé, Vincent
dc.date.accessioned2014-06-12T13:15:28Z
dc.date.available2014-06-12T13:15:28Z
dc.date.issued2011-05
dc.date.updated2014-06-03T09:44:06Z
dc.description.abstractFish have recently been recognized as a suitable model and a promising alternative to mammalian systems to study skeletogenesis. In this regard, several fish bone-derived cell lines have been developed and are being used to investigate mechanisms associated with insulin-like action of vanadium on extracellular matrix (ECM) mineralization. Although proliferative and mineralogenic effects of vanadate, insulin-like growth factor 1 (IGF-1), and insulin have recently been evaluated in a fish prechondrocyte cell line, no data are available in fish bone-forming cells, the osteoblasts. Using fish preosteoblast cells, we showed that IGF-1, but not insulin or vanadate, stimulated cell proliferation through the mitogen-activated protein kinase (MAPK) pathway, while both IGF-1 and vanadate inhibited cell differentiation/ECM mineralization through the same mechanism. Our data also indicated that the phosphatidyl inositol-3 kinase (PI-3K) pathway stimulates differentiation/ECM mineralization in osteoblasts and could represent a way to balance MAPK pathway action. The comparison of these new data obtained in fish with those available in mammals clearly evidenced a conservation of regulatory mechanisms among vertebrate bone-derived systems, although different players are involved.por
dc.identifier.citationTiago, D.M.; Cancela, M.L.; Laizé, V.Proliferative and mineralogenic effects of insulin, IGF-1, and vanadate in fish osteoblast-like cells, Journal of Bone and Mineral Metabolism, 29, 3, 377-382, 2011.por
dc.identifier.doihttp://doi:10.1152/ 10.1007/s00774-010-0243-7
dc.identifier.issn0914-8779
dc.identifier.otherAUT: LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/4280
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringer Verlagpor
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs00774-010-0243-7por
dc.subjectVanadiumpor
dc.subjectInsulin-like activitypor
dc.subjectIntracellular signaling pathwaypor
dc.subjectIn vitro mineralizationpor
dc.subjectBony fish osteoblastpor
dc.titleProliferative and mineralogenic effects of insulin, IGF-1, and vanadate in fish osteoblast-like cellspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage382por
oaire.citation.issue3por
oaire.citation.startPage377por
oaire.citation.titleJournal of Bone and Mineral Metabolismpor
oaire.citation.volume29por
person.familyNameTiago
person.familyNameCancela
person.familyNameLaizé
person.givenNameDaniel
person.givenNameM. Leonor
person.givenNameVincent
person.identifier.orcid0000-0001-8418-6292
person.identifier.orcid0000-0003-3114-6662
person.identifier.orcid0000-0001-9565-9198
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id14422711000
person.identifier.scopus-author-id6602982778
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication10cda3a0-7b9c-4d16-bd2a-a06df1d56d94
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublication.latestForDiscovery10cda3a0-7b9c-4d16-bd2a-a06df1d56d94

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Proliferative and mineralogenic effects of insulin, IGF-1, and vanadate in fish osteoblast-like cells.pdf
Size:
303.34 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)