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O impacto do zinco no processo de cicatrizaĆ§Ć£o e envelhecimento da pele
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Abstract(s)
A luta contra o envelhecimento Ć© um grande desafio no sĆ©culo XXI, especialmente na sua aĆ§Ć£o sobre a pele. A mudanƧa nas propriedades biomecĆ¢nicas das cĆ©lulas cutĆ¢neas, particularmente dos fibroblastos dĆ©rmicos, estĆ” relacionada Ć perda de tecido cutĆ¢neo, causando rugas e outros sinais visĆveis. Durante o envelhecimento, a derme sofre uma reduĆ§Ć£o na produĆ§Ć£o de colagĆ©nio e elastina, levando a uma diminuiĆ§Ć£o na produĆ§Ć£o da matriz extracelular e na eficiĆŖncia dos fibroblastos.
A pele envelhecida possui menos miofibroblastos ativos, reduzindo a sua capacidade de remodelaĆ§Ć£o da matriz extracelular. Isso resulta numa cicatrizaĆ§Ć£o menos eficiente, podendo atĆ© levar ao aparecimento de patologias da pele mais graves. Portanto, uma melhor compreensĆ£o dos mecanismos envolvidos nestes processos Ć© necessĆ”ria para desenvolver produtos eficazes que reduzam os sinais visĆveis do envelhecimento.
O zinco desempenha um papel crucial na cicatrizaĆ§Ć£o, uma vez que, os seus iƵes sĆ£o essenciais para a homeostasia da pele e tĆŖm efeitos benĆ©ficos na cicatrizaĆ§Ć£o de tecidos danificados, devido ao seu papel em vĆ”rios processos metabĆ³licos e na regulaĆ§Ć£o de enzimas envolvidas na reparaĆ§Ć£o do ADN e na matriz extracelular.
O presente trabalho consistirĆ” em duas partes distintas: na primeira, uma pesquisa bibliogrĆ”fica para resumir o estado da arte sobre o zinco, as suas propriedades e aplicaƧƵes como um potencial agente de antienvelhecimento, bem como a sua importĆ¢ncia no processo de cicatrizaĆ§Ć£o. A segunda parte serĆ” focada em ensaios laboratoriais in vitro, utilizando uma linha celular de fibroblastos BJ-5ta para investigar o papel do zinco nesse processo.
A discussĆ£o final abordarĆ” as minhas conclusƵes da pesquisa bibliogrĆ”fica e dos resultados laboratoriais, incluindo as tĆ©cnicas usadas, plano experimental, ensaios e anĆ”lise dos dados, sempre alinhados com os objetivos estabelecidos.
The battle against ageing is a major challenge in the 21st century, especially in its relation to the skin. The change in the biomechanical properties of skin cells, particularly dermal fibroblasts, is related to the loss of skin tissue, causes wrinkles and other visible signs of aging. During aging, there is a reduction in extracellular matrix production reflected in less collagen and elastin in the dermis, due to the diminished function of fibroblasts. Furthermore, aging skin has fewer activated myofibroblasts, which means there is a reduced ability to remodel the extracellular matrix. This results in less efficient healing and may even lead to the development of more serious skin pathologies. Therefore, a better understanding of the mechanisms involved in skin-related processes is necessary to develop effective derma cosmeceuticals that ensure skin health and reduce visible signs of aging. Zinc plays a crucial role in wound healing, since its ions are essential for maintaining skin homeostasis and have beneficial effects on the healing of damaged tissues, due to their role in various metabolic processes and in the regulation of enzymes involved in DNA repair and the extracellular matrix. This thesis project consists of two distinct parts: first, a literature review to summarize the state of the art about zinc, its properties, its applications for skin pathologies and its potential as an anti-aging agent. The second part of the thesis focusses on in vitro laboratory trials, and assesses the effect of zinc on, BJ-5ta, a fibroblast cell line derived from skin. The final discussion presents my conclusions about the use of zinc as an anti-aging agent based on the literature review and the laboratory results obtained, including the techniques used, experimental plan, tests, and data analysis.
The battle against ageing is a major challenge in the 21st century, especially in its relation to the skin. The change in the biomechanical properties of skin cells, particularly dermal fibroblasts, is related to the loss of skin tissue, causes wrinkles and other visible signs of aging. During aging, there is a reduction in extracellular matrix production reflected in less collagen and elastin in the dermis, due to the diminished function of fibroblasts. Furthermore, aging skin has fewer activated myofibroblasts, which means there is a reduced ability to remodel the extracellular matrix. This results in less efficient healing and may even lead to the development of more serious skin pathologies. Therefore, a better understanding of the mechanisms involved in skin-related processes is necessary to develop effective derma cosmeceuticals that ensure skin health and reduce visible signs of aging. Zinc plays a crucial role in wound healing, since its ions are essential for maintaining skin homeostasis and have beneficial effects on the healing of damaged tissues, due to their role in various metabolic processes and in the regulation of enzymes involved in DNA repair and the extracellular matrix. This thesis project consists of two distinct parts: first, a literature review to summarize the state of the art about zinc, its properties, its applications for skin pathologies and its potential as an anti-aging agent. The second part of the thesis focusses on in vitro laboratory trials, and assesses the effect of zinc on, BJ-5ta, a fibroblast cell line derived from skin. The final discussion presents my conclusions about the use of zinc as an anti-aging agent based on the literature review and the laboratory results obtained, including the techniques used, experimental plan, tests, and data analysis.
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Keywords
Pele Envelhecimento CĆ©lulas bj-5ta CicatrizaĆ§Ć£o Zinco TĆ©cnicas laboratoriais