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O impacto do zinco no processo de cicatrização e envelhecimento da pele
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Abstract(s)
A luta contra o envelhecimento é um grande desafio no século XXI, especialmente na sua ação sobre a pele. A mudança nas propriedades biomecânicas das células cutâneas, particularmente dos fibroblastos dérmicos, está relacionada à perda de tecido cutâneo, causando rugas e outros sinais visíveis. Durante o envelhecimento, a derme sofre uma redução na produção de colagénio e elastina, levando a uma diminuição na produção da matriz extracelular e na eficiência dos fibroblastos.
A pele envelhecida possui menos miofibroblastos ativos, reduzindo a sua capacidade de remodelação da matriz extracelular. Isso resulta numa cicatrização menos eficiente, podendo até levar ao aparecimento de patologias da pele mais graves. Portanto, uma melhor compreensão dos mecanismos envolvidos nestes processos é necessária para desenvolver produtos eficazes que reduzam os sinais visíveis do envelhecimento.
O zinco desempenha um papel crucial na cicatrização, uma vez que, os seus iões são essenciais para a homeostasia da pele e têm efeitos benéficos na cicatrização de tecidos danificados, devido ao seu papel em vários processos metabólicos e na regulação de enzimas envolvidas na reparação do ADN e na matriz extracelular.
O presente trabalho consistirá em duas partes distintas: na primeira, uma pesquisa bibliográfica para resumir o estado da arte sobre o zinco, as suas propriedades e aplicações como um potencial agente de antienvelhecimento, bem como a sua importância no processo de cicatrização. A segunda parte será focada em ensaios laboratoriais in vitro, utilizando uma linha celular de fibroblastos BJ-5ta para investigar o papel do zinco nesse processo.
A discussão final abordará as minhas conclusões da pesquisa bibliográfica e dos resultados laboratoriais, incluindo as técnicas usadas, plano experimental, ensaios e análise dos dados, sempre alinhados com os objetivos estabelecidos.
The battle against ageing is a major challenge in the 21st century, especially in its relation to the skin. The change in the biomechanical properties of skin cells, particularly dermal fibroblasts, is related to the loss of skin tissue, causes wrinkles and other visible signs of aging. During aging, there is a reduction in extracellular matrix production reflected in less collagen and elastin in the dermis, due to the diminished function of fibroblasts. Furthermore, aging skin has fewer activated myofibroblasts, which means there is a reduced ability to remodel the extracellular matrix. This results in less efficient healing and may even lead to the development of more serious skin pathologies. Therefore, a better understanding of the mechanisms involved in skin-related processes is necessary to develop effective derma cosmeceuticals that ensure skin health and reduce visible signs of aging. Zinc plays a crucial role in wound healing, since its ions are essential for maintaining skin homeostasis and have beneficial effects on the healing of damaged tissues, due to their role in various metabolic processes and in the regulation of enzymes involved in DNA repair and the extracellular matrix. This thesis project consists of two distinct parts: first, a literature review to summarize the state of the art about zinc, its properties, its applications for skin pathologies and its potential as an anti-aging agent. The second part of the thesis focusses on in vitro laboratory trials, and assesses the effect of zinc on, BJ-5ta, a fibroblast cell line derived from skin. The final discussion presents my conclusions about the use of zinc as an anti-aging agent based on the literature review and the laboratory results obtained, including the techniques used, experimental plan, tests, and data analysis.
The battle against ageing is a major challenge in the 21st century, especially in its relation to the skin. The change in the biomechanical properties of skin cells, particularly dermal fibroblasts, is related to the loss of skin tissue, causes wrinkles and other visible signs of aging. During aging, there is a reduction in extracellular matrix production reflected in less collagen and elastin in the dermis, due to the diminished function of fibroblasts. Furthermore, aging skin has fewer activated myofibroblasts, which means there is a reduced ability to remodel the extracellular matrix. This results in less efficient healing and may even lead to the development of more serious skin pathologies. Therefore, a better understanding of the mechanisms involved in skin-related processes is necessary to develop effective derma cosmeceuticals that ensure skin health and reduce visible signs of aging. Zinc plays a crucial role in wound healing, since its ions are essential for maintaining skin homeostasis and have beneficial effects on the healing of damaged tissues, due to their role in various metabolic processes and in the regulation of enzymes involved in DNA repair and the extracellular matrix. This thesis project consists of two distinct parts: first, a literature review to summarize the state of the art about zinc, its properties, its applications for skin pathologies and its potential as an anti-aging agent. The second part of the thesis focusses on in vitro laboratory trials, and assesses the effect of zinc on, BJ-5ta, a fibroblast cell line derived from skin. The final discussion presents my conclusions about the use of zinc as an anti-aging agent based on the literature review and the laboratory results obtained, including the techniques used, experimental plan, tests, and data analysis.
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Keywords
Pele Envelhecimento Células bj-5ta Cicatrização Zinco Técnicas laboratoriais