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Digestive and metabolic consequences of on-growing greater amberjack (Seriola dumerili) juveniles at different temperatures. In-vivo and ex-vivo assessment

2025-03Journal article Open access
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dc.contributor.authorNavarro-Guillén, C.
dc.contributor.authorPerera, E.
dc.contributor.authorPérez-Hilario, D.
dc.contributor.authorMartos-Sitcha, J.A.
dc.contributor.authorMolina-Roque, L.
dc.contributor.authorGregorio, Silvia
dc.contributor.authorFonseca, Filomena
dc.contributor.authorFuentes, J.
dc.contributor.authorYúfera, M.
dc.date.accessioned2025-01-29T12:59:11Z
dc.date.available2025-01-29T12:59:11Z
dc.date.issued2025-03
dc.description.abstractAiming to elucidate the effects of temperature on different aspects of the fish digestive physiology that may affect the growth, we examined, firstly, variations in growth performance, feed intake, digestive enzyme activities, expression of transport related genes, metabolites in plasma and liver, and oxidative stress response in juveniles of greater amberjack (Seriola dumerili) growing during two months at 18, 22 and 26 degrees C of water temperature. Secondly, we analysed the epithelial function of the intestinal mucosa by electrophysiological ex-vivo experiments. As expected, body growth increased with increasing temperature in the tested range. Highest relative feed intake was observed at 26 degrees C, but the food conversion ratio was the same at 22 and 26 degrees C and less favourable at 18 degrees C. Digestive proteases activities were similar at 22 and 26 degrees C, while an evident effect of temperature was observed on lipids digestive capacity, being lipase activity undetectable at 18 degrees C. Electrophysiological assays revealed a relationship between temperature and intestinal mucosa plasticity. Temperature increase promoted epithelial functionality through higher tissue resistance and short-circuit current in mid-intestine at 26 degrees C, as well as better electrogenic amino acids transport. On the other hand, mRNA expression of peptide transporters tended to be higher in fish that grew at 18 degrees C, probably to reinforce the transport capacity. Plasma circulating levels of metabolites demonstrated higher energy and protein mobilization with the increasing temperature, where a hypometabolic state was denoted by lower cortisol levels at 18 degrees C together with an apparent switch from lipids to carbohydrate usage as energy source and increased oxidative stress in the liver at the lowest temperature. Altogether indicates that the tested temperatures are within the tolerance range for the species, although 22 and 26 degrees C appear as optimal temperatures for on-growing greater amberjack juveniles. Changes in nutrient digestion and absorption induced by temperature are related with both hydrolytic activity and remodelling of the intestinal mucosa. Impairing growth capacity and initial evidences of welfare compromise were observed at 18 degrees C.eng
dc.description.sponsorshipDOC_01203; RTI2018-096134-B-I00
dc.identifier.doi10.1016/j.aquaculture.2024.742011
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10400.1/26691
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationCentre for Marine and Environmental Research (CIMA)
dc.relationAquatic Research Infrastructure Network
dc.relation.ispartofAquaculture
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectDigestion
dc.subjectIntestinal integrity
dc.subjectTemperature
dc.subjectUssing chambers
dc.subjectGreater amberjack
dc.titleDigestive and metabolic consequences of on-growing greater amberjack (Seriola dumerili) juveniles at different temperatures. In-vivo and ex-vivo assessmenteng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Marine and Environmental Research (CIMA)
oaire.awardTitleAquatic Research Infrastructure Network
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00350%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0069%2F2020/PT
oaire.citation.startPage742011
oaire.citation.titleAquaculture
oaire.citation.volume598
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameGregorio
person.familyNameFonseca
person.givenNameSilvia
person.givenNameFilomena
person.identifier1579837
person.identifier.ciencia-idBF14-020B-4C39
person.identifier.orcid0000-0002-6648-5122
person.identifier.orcid0000-0003-3824-0459
person.identifier.ridG-1618-2018
person.identifier.scopus-author-id7004434992
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublication9bc940d8-7dfb-4950-98f4-6853f398cb49
relation.isAuthorOfPublication8eb52d8c-f5c0-4ac5-9dbb-a985321045a3
relation.isAuthorOfPublication.latestForDiscovery9bc940d8-7dfb-4950-98f4-6853f398cb49
relation.isProjectOfPublication62b4d568-8b21-464c-97ed-de826eab4136
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relation.isProjectOfPublication.latestForDiscovery62b4d568-8b21-464c-97ed-de826eab4136

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