Repository logo
 
Publication

Proteomics of sea bass skin-scales exposed to the emerging pollutant fluoxetine compared to estradiol

2022Journal article Restricted access
Simple item page
dc.contributor.authorAnjos, L.
dc.contributor.authorEstêvão, Dulce
dc.contributor.authorSantos, S.
dc.contributor.authorSanta, C.
dc.contributor.authorManadas, B.
dc.contributor.authorMonsinjon, T.
dc.contributor.authorCanario, Adelino
dc.contributor.authorPower, Deborah
dc.contributor.authorPinto, Patricia IS
dc.date.accessioned2022-03-11T15:11:51Z
dc.date.available2022-03-11T15:11:51Z
dc.date.issued2022
dc.description.abstractTeleost fish skin-scales are essential for protection and homeostasis and the largest tissue in direct contact with the environment, but their potential as early indicators of pollutant exposure are hampered by limited knowledge about this model. This study evaluated multi-level impacts of in vivo exposure of European sea bass to fluoxetine (FLX, a selective serotonin-reuptake inhibitor and an emerging pollutant) and 17 beta-estradiol (E2, a natural hormone and representative of diverse estrogenic endocrine-disrupting pollutants). Exposed fish had significantly increased circulating levels of FLX and its active metabolite nor-FLX that, in contrast to E2, did not have estrogenic effects on most fish plasma and scale indicators. Quantitative proteomics using SWATH-MS identified 985 proteins in the scale total proteome. 213 proteins were significantly modified 5 days after exposure to E2 or FLX and 31 were common to both treatments and responded in the same way. Common biological processes significantly affected by both treatments were protein turnover and cytoskeleton reorganization. E2 specifically up-regulated proteins related to protein production and degradation and down-regulated the cytoskeleton/extracellular matrix and innate immune proteins. FLX caused both up- and down-regulation of protein synthesis and energy metabolism. Multiple estrogen and serotonin receptor and transporter transcripts were altered in sea bass scales after E2 and/or FLX exposure, revealing complex disruptive effects in estrogen/serotonin responsiveness, which may account for the partially overlapping effects of E2 and FLX on the proteome. A large number (103) of FLX-specifically regulated proteins indicated numerous actions independent of estrogen signalling. This study provides the first quantitative proteome of the fish skin-scale barrier, elucidates routes of action and biochemical and molecular signatures of E2 or FLX-exposure and identifies potential physiological consequences and candidate biomarkers of pollutant exposure, for monitoring and risk assessment.pt_PT
dc.description.sponsorshipPOCI01-0145-FEDER-402-022125; EMBRC.PT ALG-01-0145-FEDER-022121; POCI-01-0145-FEDER-007440; ROTEIRO/0028/2013
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.scitotenv.2021.152671pt_PT
dc.identifier.issn0048-9697
dc.identifier.urihttp://hdl.handle.net/10400.1/17675
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationE2SCALE: Scaling-down estrogenic responses in fish
dc.relationAlgarve Centre for Marine Sciences
dc.relationCenter for Innovative Biomedicine and Biotechnology
dc.relationNot Available
dc.relationEuropean Marine Biological Research Infrastructure Cluster to promote the Blue Bioeconomy
dc.subjectEstrogenpt_PT
dc.subjectFish scalespt_PT
dc.subjectQuantitative proteomicspt_PT
dc.subjectSSRI antidepressantpt_PT
dc.subjectTeleost fishpt_PT
dc.titleProteomics of sea bass skin-scales exposed to the emerging pollutant fluoxetine compared to estradiolpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleE2SCALE: Scaling-down estrogenic responses in fish
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCenter for Innovative Biomedicine and Biotechnology
oaire.awardTitleNot Available
oaire.awardTitleEuropean Marine Biological Research Infrastructure Cluster to promote the Blue Bioeconomy
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAAG-GLO%2F4003%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04539%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL 57%2F2016%2FCP1361%2FCT0015/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/654008/EU
oaire.citation.startPage152671pt_PT
oaire.citation.titleScience of The Total Environmentpt_PT
oaire.citation.volume814pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamDL 57/2016
oaire.fundingStreamH2020
person.familyNameEstêvão
person.familyNameCanario
person.familyNamePower
person.familyNamePinto
person.givenNameMaria Dulce da Mota Antunes de Oliveira
person.givenNameAdelino
person.givenNameDeborah Mary
person.givenNamePatricia IS
person.identifierI-4695-2014
person.identifier143624
person.identifier643457
person.identifier.ciencia-id881B-53E8-0DB1
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.orcid0000-0002-7151-8363
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0001-7854-3898
person.identifier.ridC-7942-2009
person.identifier.ridM-3817-2013
person.identifier.scopus-author-id8381590400
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id10240774300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication67887883-2695-48cc-8221-aa46780ac143
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication64bc526e-5281-44e5-91eb-5743436109f9
relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isProjectOfPublication66289c1f-16cd-4d25-b30f-1eb63d5ecd80
relation.isProjectOfPublicationfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f
relation.isProjectOfPublication428f397e-22dd-493a-b097-988a1717f76f
relation.isProjectOfPublicationf0b2c972-aaf9-49e8-a11a-5b9f377b27a3
relation.isProjectOfPublicationd5cd89fe-f500-412a-ab83-8d5c84eed8b1
relation.isProjectOfPublication.latestForDiscoveryd5cd89fe-f500-412a-ab83-8d5c84eed8b1

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
1-s2.0-S0048969721077494-main.pdf
Size:
2.28 MB
Format:
Adobe Portable Document Format
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)
ESS2-Artigos (em revistas ou actas indexadas)