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Isolation and selection of protein-rich mutants of chlorella vulgaris by fluorescence-activated cell sorting with enhanced biostimulant activity to germinate garden cress seeds

2024-09-01Journal article Open access
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dc.contributor.authorTrovão dos Santos, Mafalda
dc.contributor.authorSchüler, Lisa
dc.contributor.authorPedroso, Humberto
dc.contributor.authorReis, Ana
dc.contributor.authorSanto, Gonçalo Espírito
dc.contributor.authorBarros, Ana
dc.contributor.authorCorreia, Nádia
dc.contributor.authorRibeiro, Joana
dc.contributor.authorBombo, Gabriel
dc.contributor.authorGama, Florinda
dc.contributor.authorViana, Catarina
dc.contributor.authorCosta, Monya
dc.contributor.authorFerreira, Sara
dc.contributor.authorCardoso, Helena
dc.contributor.authorVarela, João
dc.contributor.authorSilva, Joana
dc.contributor.authorFreitas, Filomena
dc.contributor.authorPereira, Hugo
dc.date.accessioned2024-10-18T08:52:00Z
dc.date.available2024-10-18T08:52:00Z
dc.date.issued2024-09-01
dc.description.abstractMicroalgae are a promising feedstock with proven biostimulant activity that is enhanced by their biochemical components (e.g., amino acids and phytohormones), which turns them into an appealing feedstock to reduce the use of fertilisers in agriculture and improve crop productivity and resilience. Thus, this work aimed to isolate protein-rich microalgal mutants with increased biostimulant activity. Random mutagenesis was performed with Chlorella vulgaris, and a selection of protein-rich mutants were sorted through fluorescence-activated cell sorting (FACS), resulting in the isolation of 17 protein-rich mutant strains with protein contents 19-34% higher than that of the wildtype (WT). Furthermore, mutant F4 displayed a 38%, 22% and 62% higher biomass productivity, growth rate and chlorophyll content, respectively. This mutant was then scaled up to a 7 L benchtop reactor to produce biomass and evaluate the biostimulant potential of this novel strain towards garden cress seeds. Compared to water (control), the germination index and the relative total growth increased by 7% and 19%, respectively, after the application of 0.1 g L-1 of this bioproduct, which highlights its biostimulant potential.eng
dc.description.sponsorshipALG-01-0247-FEDER-069961 PERFORMALGAE; 2020.06814; 2020.07229
dc.identifier.doi10.3390/plants13172441
dc.identifier.issn2223-7747
dc.identifier.urihttp://hdl.handle.net/10400.1/26110
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationApplied Molecular Biosciences Unit
dc.relationApplied Molecular Biosciences Unit
dc.relationInstitute for Health and Bioeconomy
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relation.ispartofPlants
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBiostimulants
dc.subjectFluorescence-activated cell sorting
dc.subjectMicroalgae
dc.subjectProtein
dc.subjectRandom mutagenesis
dc.subjectSelection method
dc.titleIsolation and selection of protein-rich mutants of chlorella vulgaris by fluorescence-activated cell sorting with enhanced biostimulant activity to germinate garden cress seedseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleInstitute for Health and Bioeconomy
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.issue17
oaire.citation.startPage2441
oaire.citation.titlePlants
oaire.citation.volume13
oaire.fundingStream6817 - DCRRNI ID
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oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.affiliation.nameUniversidade do Algarve
person.familyNameTrovão dos Santos
person.familyNameSchüler
person.familyNameBombo
person.familyNameGama
person.familyNameViana
person.familyNameCosta
person.familyNameFerreira
person.familyNameVarela
person.familyNameSilva
person.familyNamePereira
person.givenNameMafalda
person.givenNameLisa
person.givenNameGabriel
person.givenNameFlorinda
person.givenNameCatarina
person.givenNameMonya
person.givenNameSara
person.givenNameJoão
person.givenNameJoana
person.givenNameHugo
person.identifier282781
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person.identifier.ciencia-id331E-84FD-2F34
person.identifier.orcid0000-0002-9465-7681
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person.identifier.orcid0000-0003-3976-7834
person.identifier.orcid0000-0003-3101-693X
person.identifier.orcid0000-0003-3977-7418
person.identifier.orcid0000-0002-1369-2099
person.identifier.ridM-4223-2013
person.identifier.ridE-9360-2015
person.identifier.scopus-author-id35770492700
person.identifier.scopus-author-id3473062
person.identifier.scopus-author-id8094380800
person.identifier.scopus-author-id55741416000
person.identifier.scopus-author-id54080259900
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project.funder.nameFundação para a Ciência e a Tecnologia
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