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Adaptation to different salinities exposes functional specialization in the intestine of the sea bream (Sparus aurata L.)

2013Journal article Open access
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dc.contributor.authorGregorio, Silvia
dc.contributor.authorCarvalho, Edison Samir Mascarelhas
dc.contributor.authorEncarnação, Sandra
dc.contributor.authorWilson, J.
dc.contributor.authorPower, Deborah
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorFuentes, J.
dc.date.accessioned2013-11-29T15:44:22Z
dc.date.available2013-11-29T15:44:22Z
dc.date.issued2013
dc.date.updated2013-11-29T15:22:17Z
dc.description.abstractThe processing of intestinal fluid, in addition to a high drinking rate, is essential for osmoregulation in marine fish. This study analyzed the long-term response of the sea bream (Sparus aurata L.) to relevant changes of external salinity (12, 35 and 55p.p.t.), focusing on the anterior intestine and in the less-often studied rectum. Intestinal water absorption, epithelial HCO3– secretion and gene expression of the main molecular mechanisms (SLC26a6, SLC26a3, SLC4a4, atp6v1b, CFTR, NKCC1 and NKCC2) involved in Cl– and HCO3– movements were examined. The anion transporters SLC26a6 and SLC26a3 are expressed severalfold higher in the anterior intestine, while the expression of Atp6v1b (V-type H+-ATPase β-subunit) is severalfold higher in the rectum. Prolonged exposure to altered external salinity was without effect on water absorption but was associated with concomitant changes in intestinal fluid content, epithelial HCO3– secretion and salinity-dependent expression of SLC26a6, SLC26a3 and SLC4a4 in the anterior intestine. However, the most striking response to external salinity was obtained in the rectum, where a 4- to 5-fold increase in water absorption was paralleled by a 2- to 3-fold increase in HCO3– secretion in response to a salinity of 55p.p.t. In addition, the rectum of high salinity-acclimated fish shows a sustained (and enhanced) secretory current (Isc), identified in vitro in Ussing chambers and confirmed by the higher expression of CFTR and NKCC1 and by immunohistochemical protein localization. Taken together, the present results suggest a functional anterior–posterior specialization with regard to intestinal fluid processing and subsequently to salinity adaptation of the sea bream. The rectum becomes more active at higher salinities and functions as the final controller of intestinal function in osmoregulation.por
dc.identifier.citationGregorio, Slvia F.; Carvalho, Edison S. M.; Encarnacao, Sandra; Wilson, Jonathan M.; Power, Deborah M.; Canario, Adelino V. M.; Fuentes, Juan. Adaptation to different salinities exposes functional specialization in the intestine of the sea bream (Sparus aurata L.), Journal of Experimental Biology, 216, 3, 470-479, 2013.por
dc.identifier.otherAUT: DPO00386; ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3175
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherCompany of Biologistspor
dc.relation.publisherversionhttp://jeb.biologists.orgpor
dc.titleAdaptation to different salinities exposes functional specialization in the intestine of the sea bream (Sparus aurata L.)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage479por
oaire.citation.issue3por
oaire.citation.startPage470por
oaire.citation.titleJournal of Experimental Biologypor
oaire.citation.volume216por
person.familyNameGregorio
person.familyNamePower
person.familyNameCanario
person.familyNameFuentes
person.givenNameSilvia
person.givenNameDeborah Mary
person.givenNameAdelino
person.givenNameJuan
person.identifier1579837
person.identifier143624
person.identifier392998
person.identifier.ciencia-idBF14-020B-4C39
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id421B-E196-2C33
person.identifier.orcid0000-0002-6648-5122
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-0430-8734
person.identifier.ridG-1618-2018
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7201832526
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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