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Dietary lysine imbalance affects muscle proteome in zebrafish (Danio rerio): a comparative 2D-DIGE study

2012Journal article Restricted access
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dc.contributor.authorVareilles, Mahaut
dc.contributor.authorConceição, L. E. C.
dc.contributor.authorGómez-Requeni, Pedro
dc.contributor.authorKousoulaki, Katerina
dc.contributor.authorRichard, Nadège
dc.contributor.authorRodrigues, Pedro
dc.contributor.authorFladmark, K. E.
dc.contributor.authorRønnestad, I.
dc.date.accessioned2014-06-17T15:45:03Z
dc.date.available2014-06-17T15:45:03Z
dc.date.issued2012
dc.date.updated2014-06-12T10:16:18Z
dc.description.abstractLysine (Lys) is an indispensable amino acid (AA) and generally the first limiting AA in vegetable protein sources in fish feeds. Inadequate dietary Lys availability may limit protein synthesis, accretion and growth of fish. This experiment aimed to further elucidate the role of Lys imbalance on growth by examining the myotomal muscle proteome of juvenile zebrafish (Danio rerio). Quadruplicate groups of 8 fish were fed either a low-Lys [Lys(−), 1.34 gkg−1], medium/control (Lys, 2.47 gkg−1) or high-Lys [Lys(+), 4.63 gkg−1] diet. Fish growth was monitored from 33 to 49 days postfertilization (dpf) and trunk myotomal muscle proteome of Lys(−) and Lys(+) treatments were screened by 2D-DIGE and MALDI ToF tandem mass spectrometry. Growth rate was negatively affected by diet Lys(−). Out of 527±11 (mean±S.E.M.) protein spots detected (∼10–150 kDa and 4–7 pI value), 30 were over-expressed and 22 under-expressed in Lys(−) fish (|fold-change|>1.2, p value <0.05). Higher myosin light chains abundance and other myofibrillar proteins in Lys (−) fish pointed to increased sarcomeric degradation, indicating a higher protein turnover for supplying basal energysaving metabolism rather than growth and muscle protein accretion. The Lys deficiency also possibly induced a higher feeding activity, reflected in the over-expression of beta enolase and mitochondrial ATP synthase. Contrarily, in the faster growing fish [Lys(+)], over-expression of apolipoprotein A-I, F-actin capping protein and Pdlim7 point to increased energy storage as fat and enhanced muscle growth, particularly by mosaic hyperplasia. Thus using an exploratory approach, this study pinpoints interesting candidates for further elucidating the role of dietary Lys on growth of juvenile fish.por
dc.identifier.citationVareilles, Mahaut; Conceição, Luis E. C.; Gómez-Requeni, Pedro; Kousoulaki, Katerina; Richard, Nadège; Rodrigues, Pedro M.; Fladmark, Kari E.; Rønnestad, Ivar. Dietary Lysine Imbalance Affects Muscle Proteome in Zebrafish (Danio rerio): A Comparative 2D-DIGE Study, Marine Biotechnology, 14, 5, 643-654, 2012.por
dc.identifier.doihttp://dx.doi.org/10.1007/s10126-012-9462-3
dc.identifier.issn1436-2228
dc.identifier.otherAUT: PMR01268;
dc.identifier.urihttp://hdl.handle.net/10400.1/4349
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.subjectLysinepor
dc.subjectGrowthpor
dc.subjectSkeletal musclepor
dc.subjectMyosin chain isoformspor
dc.subjectPdlim7por
dc.subjectZebrafishpor
dc.titleDietary lysine imbalance affects muscle proteome in zebrafish (Danio rerio): a comparative 2D-DIGE studypor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage654por
oaire.citation.issue5por
oaire.citation.startPage643por
oaire.citation.titleMarine Biotechnologypor
oaire.citation.volume14por
person.familyNameRichard
person.familyNameRodrigues
person.givenNameNadège
person.givenNamePedro
person.identifier.orcid0000-0002-8983-0726
person.identifier.orcid0000-0002-9668-1204
person.identifier.ridM-3385-2013
person.identifier.ridM-3406-2013
person.identifier.scopus-author-id15023061500
person.identifier.scopus-author-id55107531000
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationf372969b-5422-4111-96cd-447a96f7e4f4
relation.isAuthorOfPublicationc4613fe6-e1a0-4dd9-be8d-442b2b8a7b72
relation.isAuthorOfPublication.latestForDiscoveryf372969b-5422-4111-96cd-447a96f7e4f4

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