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Cartilage Acidic Protein 2 a hyperthermostable, high affinity calcium-binding protein

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dc.contributor.authorAnjos, Liliana
dc.contributor.authorMelo, Eduardo
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-05-08T10:53:10Z
dc.date.available2014-05-08T10:53:10Z
dc.date.issued2013
dc.date.updated2014-05-08T09:34:16Z
dc.description.abstractCartilage Acidic Protein 2 (CRTAC2) is a novel protein present fromprokaryotes to vertebrateswith abundant expression in the teleost fish pituitary gland and an isoformof CRTAC1, a chondrocyte marker in humans. The two proteins are non-integrins containingN-terminal integrin-like Ca2+-bindingmotifs and their structure and function remain to be assigned. Structural studies of recombinant sea bream (sb)CRTAC2 revealed it is composed of 8.8% α-helix, 33.4% β-sheet and 57.8% unordered protein. sbCRTAC2 bound Ca2+ with high affinity (Kd= 1.46 nM) and favourable Gibbs free energy (ΔG=−12.4 kcal/mol). The stoichiometry for Ca2+ bound to sbCRTAC2 at saturation indicated six Ca2+ ligand-binding sites exist per protein molecule. No conformational change in sbCRTAC2 occurred in the presence of Ca2+. Fluorescence emission revealed that the tertiary structure of the protein is hyperthermostable between 25 °C and 95 °C and the fully unfolded state is only induced by chemical denaturing (4 MGndCl). sbCRTAC has awidespread tissue distribution and is present as highmolecular weight aggregates, although strong reducing conditions promote formation of the monomer. sbCRTAC2 promotes epithelial cell outgrowth in vitro suggesting it may share functional homology with mammalian CRTAC1, recently implicated in cell–cell and cell–matrix interactions.por
dc.identifier.citationAnjos, Liliana; Gomes, Ana S.; Melo, Eduardo P.; Canário, Adelino V.M.; Power, Deborah M. Cartilage Acidic Protein 2 a hyperthermostable, high affinity calcium-binding protein, Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 1834, 3, 642-650, 2013.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.bbapap.2012.12.012
dc.identifier.issn1570-9639
dc.identifier.otherAUT: EME01032; ACA00258; DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/3809
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationBuilding a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture
dc.subjectRecombinant Cartilage Acidic Protein 2por
dc.subjectProtein foldingpor
dc.subjectSoluble aggregatepor
dc.subjectCalciumpor
dc.subjectTeleostpor
dc.titleCartilage Acidic Protein 2 a hyperthermostable, high affinity calcium-binding proteinpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBuilding a biological knowledge-base on fish lifecycles for competitive, sustainable European aquaculture
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/222719/EU
oaire.citation.endPage650por
oaire.citation.startPage642por
oaire.citation.titleBiochimica et Biophysica Acta (BBA) - Proteins and Proteomicspor
oaire.citation.volume1834por
oaire.fundingStreamFP7
person.familyNameAnjos Guerreiro
person.familyNamePinho Melo
person.familyNameCanario
person.familyNamePower
person.givenNameLiliana Isabel Tomé
person.givenNameEduardo
person.givenNameAdelino
person.givenNameDeborah Mary
person.identifierAAK-3800-2020
person.identifier1443188
person.identifier143624
person.identifier.ciencia-id6D18-2D1C-A45C
person.identifier.ciencia-id3C1C-C10C-1510
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-9474-522X
person.identifier.orcid0000-0002-0974-8977
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id8575757700
person.identifier.scopus-author-id35566177900
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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