Protocol for isolating nuclei from frozen fish heart tissue for single-cell genomic assays

Wei, Chaoguang; Jia, Ruonan; Canario, Adelino; Chen, Liangbiao; Xu, Qianghua; Hu, Peng

http://hdl.handle.net/10400.1/27097

Utilize este identificador para referenciar este registo.

Nome:	Descrição:	Tamanho:	Formato:
1-s2.0-S2666166725001716-main.pdf		3.04 MB	Adobe PDF	Ver/Abrir

Contacte-nos

Autores

Resumo(s)

Unbiased isolation of intact single nuclei from complex tissues, such as cryopreserved hearts, for massively parallel single-cell genomic assays is particularly challenging. Here, we present a protocol for isolating high-quality nuclei from the heart tissue of Trematomus bernacchii, an Antarctic fish species with notoriously challenging tissue for nuclear extraction. We describe steps for pulverizing frozen heart tissue, library construction, and sequencing. This protocol can provide a valuable reference for isolating nuclei from other cryopreserved marine fish tissues.

URI

http://hdl.handle.net/10400.1/27097

Editora

Cell Press

DOI

10.1016/j.xpro.2025.103765

Coleções

CCM2-Artigos (em revistas ou actas indexadas)

Licença CC

cclicense-by-nc-nd

Métricas Alternativas

Ver registo completo