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Protocol for isolating nuclei from frozen fish heart tissue for single-cell genomic assays

dc.contributor.authorWei, Chaoguang
dc.contributor.authorJia, Ruonan
dc.contributor.authorCanario, Adelino
dc.contributor.authorChen, Liangbiao
dc.contributor.authorXu, Qianghua
dc.contributor.authorHu, Peng
dc.date.accessioned2025-05-09T09:51:44Z
dc.date.available2025-05-09T09:51:44Z
dc.date.issued2025-06
dc.description.abstractUnbiased isolation of intact single nuclei from complex tissues, such as cryopreserved hearts, for massively parallel single-cell genomic assays is particularly challenging. Here, we present a protocol for isolating high-quality nuclei from the heart tissue of Trematomus bernacchii, an Antarctic fish species with notoriously challenging tissue for nuclear extraction. We describe steps for pulverizing frozen heart tissue, library construction, and sequencing. This protocol can provide a valuable reference for isolating nuclei from other cryopreserved marine fish tissues.eng
dc.identifier.doi10.1016/j.xpro.2025.103765
dc.identifier.issn2666-1667
dc.identifier.urihttp://hdl.handle.net/10400.1/27097
dc.language.isoeng
dc.peerreviewedyes
dc.publisherCell Press
dc.relation.ispartofSTAR Protocols
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleProtocol for isolating nuclei from frozen fish heart tissue for single-cell genomic assayseng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue2
oaire.citation.startPage103765
oaire.citation.titleSTAR Protocols
oaire.citation.volume6
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCanario
person.givenNameAdelino
person.identifier143624
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id56568523700
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscovery5f6e51ee-9113-469e-8b9e-f30f2d452521

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