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Enzymatic capacities of metabolic fuel use in cuttlefish (Sepia officinalis) and responses to food deprivation: insight into the metabolic organization and starvation survival strategy of cephalopods

2016Journal article Restricted access
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dc.contributor.authorSpeers-Roesch, Ben
dc.contributor.authorCallaghan, Neal I.
dc.contributor.authorMacCormack, Tyson J.
dc.contributor.authorLamarre, Simon G.
dc.contributor.authorSykes, António V.
dc.contributor.authorDriedzic, William R.
dc.date.accessioned2018-11-12T20:04:12Z
dc.date.available2018-11-12T20:04:12Z
dc.date.issued2016
dc.description.abstractFood limitation is a common challenge for animals. Cephalopods are sensitive to starvation because of high metabolic rates and growth rates related to their "live fast, die young" life history. We investigated how enzymatic capacities of key metabolic pathways are modulated during starvation in the common cuttlefish (Sepia officinalis) to gain insight into the metabolic organization of cephalopods and their strategies for coping with food limitation. In particular, lipids have traditionally been considered unimportant fuels in cephalopods, yet, puzzlingly, many species (including cuttlefish) mobilize the lipid stores in their digestive gland during starvation. Using a comprehensive multi-tissue assay of enzymatic capacities for energy metabolism, we show that, during long-term starvation (12 days), glycolytic capacity for glucose use is decreased in cuttlefish tissues, while capacities for use of lipid-based fuels (fatty acids and ketone bodies) and amino acid fuels are retained or increased. Specifically, the capacity to use the ketone body acetoacetate as fuel is widespread across tissues and gill has a previously unrecognized capacity for fatty acid catabolism, albeit at low rates. The capacity for de novo glucose synthesis (gluconeogenesis), important for glucose homeostasis, likely is restricted to the digestive gland, contrary to previous reports of widespread gluconeogenesis among cephalopod tissues. Short-term starvation (3-5 days) had few effects on enzymatic capacities. Similar to vertebrates, lipid-based fuels, putatively mobilized from fat stores in the digestive gland, appear to be important energy sources for cephalopods, especially during starvation when glycolytic capacity is decreased perhaps to conserve available glucose.pt_PT
dc.description.sponsorshipSEPIATECH (31-03-05-FEP-2)
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00360-016-0991-3pt_PT
dc.identifier.issn0174-1578
dc.identifier.urihttp://hdl.handle.net/10400.1/10934
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationTowards cephalopod aquaculture: cuttlefish as the spearhead species
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAmino acidspt_PT
dc.subjectAnimalspt_PT
dc.subjectAspartate aminotransferasespt_PT
dc.subjectCitrate (si)-synthasept_PT
dc.subjectDecapodiformespt_PT
dc.subjectFatty acidspt_PT
dc.subjectFructose-bisphosphatasept_PT
dc.subjectGastrointestinal tractpt_PT
dc.subjectGluconeogenesispt_PT
dc.subjectGlucosept_PT
dc.subjectGlucose-6-phosphatasept_PT
dc.subjectGlycolysispt_PT
dc.subjectKetone bodiespt_PT
dc.subjectPhosphoenolpyruvate carboxykinase (ATP)pt_PT
dc.subjectStarvationpt_PT
dc.subjectTriglyceridespt_PT
dc.subjectEnergy metabolismpt_PT
dc.subjectCephalopodpt_PT
dc.subjectEnergy metabolismpt_PT
dc.subjectMetabolic fuel preferencept_PT
dc.subjectEnzyme activitypt_PT
dc.subjectStarvationpt_PT
dc.subjectFastingpt_PT
dc.subjectLipidpt_PT
dc.subjectKetone bodypt_PT
dc.subjectGlucosept_PT
dc.subjectDigestive glandpt_PT
dc.titleEnzymatic capacities of metabolic fuel use in cuttlefish (Sepia officinalis) and responses to food deprivation: insight into the metabolic organization and starvation survival strategy of cephalopodspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTowards cephalopod aquaculture: cuttlefish as the spearhead species
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00576%2F2014%2FCP1217%2FCT0002/PT
oaire.citation.endPage725pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage711pt_PT
oaire.citation.titleJournal of Comparative Physiology Bpt_PT
oaire.citation.volume186pt_PT
oaire.fundingStreamInvestigador FCT
person.familyNameSykes
person.givenNameAntónio
person.identifier107454
person.identifier.ciencia-id7510-6641-5A42
person.identifier.orcid0000-0002-5207-0612
person.identifier.ridC-3609-2012
person.identifier.scopus-author-id7102626426
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoveryb9d55f95-5de8-40cf-82a3-96ec98c91a55
relation.isProjectOfPublicationa7bbb7aa-912c-4d7f-933a-3905800d8dcf
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