Dopamine beta-hydroxylase and its role in regulating the growth and larval metamorphosis in Sinonovacula constricta

Li, Zhi; Niu, Donghong; Peng, Maoxiao; Xiong, Ya; Ji, Jie; Dong, Zhiguo; Li, Jiale

http://hdl.handle.net/10400.1/16492

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Dopamine beta-hydroxylase (D beta H) plays a key role in the synthesis of catecholamines (CAs) in the neuroendocrine regulatory network. The D beta H gene was identified from the razor clam Sinonovacula constricta and referred to as ScD beta H. The ScD beta H gene is a copper type II ascorbate-dependent monooxygenase with a DOMON domain and two Cu2_monooxygen domains. ScD beta H transcript expression was abundant in liver and hemolymph. During early development, ScD beta H expression significantly increased at the umbo larval stage. Furthermore, the inhibitors and siRNA of D beta H were screened. After challenge with D beta H inhibitor, the larval metamorphosis and survival rates, and juvenile growth were obviously decreased. Under the siRNA stress, the larval metamorphosis and survival rates were also significantly decreased. Therefore, ScD beta H may play an important regulating role in larval metamorphosis and juvenile growth.