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Identification of an osteopontin-like protein in fish associated with mineral formation

2007Journal article Restricted access
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dc.contributor.authorFonseca, V. G.
dc.contributor.authorLaizé, Vincent
dc.contributor.authorValente, M. S.
dc.contributor.authorCancela, Leonor
dc.date.accessioned2014-06-17T12:59:34Z
dc.date.available2014-06-17T12:59:34Z
dc.date.issued2007
dc.date.updated2014-06-03T09:54:13Z
dc.description.abstractFish has been recently recognized as a suitable vertebrate model and represents a promising alternative to mammals for studying mechanisms of tissue mineralization and unravelling specific questions related to vertebrate bone formation. The recently developed Sparus aurata (gilthead seabream) osteoblast-like cell line VSa16 was used to construct a cDNA subtractive library aimed at the identification of genes associated with fish tissue mineralization. Suppression subtractive hybridization, combined with mirror orientation selection, identified 194 cDNA clones representing 20 different genes up-regulated during the mineralization of the VSa16 extracellular matrix. One of these genes accounted for 69% of the total number of clones obtained and was later identified as theS. aurata osteopontin-like gene. The 2138-bp full-length S. aurata osteopontin-like cDNA was shown to encode a 374 amino-acid protein containing domains and motifs characteristic of osteopontins, such as an integrin receptor-binding RGD motif, a negatively charged domain and numerous post-translational modifications (e.g. phosphorylations and glycosylations). The common origin of mammalian osteopontin and fish osteopontin-like proteins was indicated through an in silico analysis of available sequences showing similar gene and protein structures and was further demonstrated by their specific expression in mineralized tissues and cell cultures. Accordingly, and given its proven association with mineral formation and its characteristic protein domains, we propose that the fish osteopontin-like protein may play a role in hard tissue mineralization, in a manner similar to osteopontin in higher vertebrates.por
dc.identifier.citationFonseca VG; Laizé V; Valente MS; Cancela ML. Identification of an osteopontin-like protein in fish associated with mineral formation, FEBS Journal, 274, 17, 4428-4439, 2007.por
dc.identifier.doihttp://dx.doi.org/10.1111/j.1742-4658.2007.05972.x
dc.identifier.issn1742-464X
dc.identifier.otherAUT: LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/4337
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1111/j.1742-4658.2007.05972.x/abstract;jsessionid=9E201AFA8277F92BA294B690BB979934.f02t01por
dc.subjectBone-derived cell linepor
dc.subjectGilthead seabream Sparus aurata (Teleostei)por
dc.subjectOsteopontinpor
dc.subjectSubtractive librarypor
dc.subjectTissue mineralizationpor
dc.titleIdentification of an osteopontin-like protein in fish associated with mineral formationpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage4439por
oaire.citation.issue17por
oaire.citation.startPage4428por
oaire.citation.titleFEBS Journalpor
oaire.citation.volume247por
person.familyNameLaizé
person.familyNameCancela
person.givenNameVincent
person.givenNameM. Leonor
person.identifier.orcid0000-0001-9565-9198
person.identifier.orcid0000-0003-3114-6662
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id6602982778
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublication.latestForDiscoveryb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f

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