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In vitro propagation of Limonium algarvense Erben: towards the conservation of a rare and endemic halophyte

2025-06-23Artigo científico Acesso restrito
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datacite.subject.sdg15:Proteger a Vida Terrestre
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
datacite.subject.sdg13:Ação Climática
dc.contributor.authorCustódio, Luísa
dc.contributor.authorLescano, Leonardo
dc.contributor.authorMartins, Carolina
dc.contributor.authorRodrigues, Maria João
dc.date.accessioned2026-04-13T12:44:43Z
dc.date.available2026-04-13T12:44:43Z
dc.date.issued2025-06-23
dc.description.abstractLimonium algarvense, an endemic halophyte from the Southwest Iberian Peninsula, is listed as “Near Threatened” due to habitat degradation caused by climate change and human activities. Its medicinal properties and limited natural propagation make conservation efforts essential. In vitro propagation offers a sustainable ex situ conservation strategy for this species. Shoot tips from greenhouse-germinated seedlings were sterilized and cultured on Murashige and Skoog (MS) medium supplemented with various growth regulators for 2 cycles of multiplication, such as kinetin (KIN) and naphthalene acetic acid (NAA). Rooting was induced using various MS-based media, including supplements like calcium chloride (CaCl₂) and activated charcoal (AC), followed by a gradual acclimatization to ex vitro conditions. Aseptic cultures were successfully established in 75.6% of the explants using 0.1% HgCl₂. The optimal combination for shoot multiplication was 2.5 mg/L KIN+0.1 mg/L NAA, producing 15.7 shoots/explant after two cycles. The highest rooting percentage (67.6%) was obtained with MS medium supplemented with 10 mM CaCl₂, which also led to superior root length, shoot height, and rosette diameter compared to other treatments. However, survival during the acclimatization remained low (13%). This highlights the need for further optimization of the acclimatization phase to improve the ex vitro survival of propagated plants. Overall, the in vitro protocol provides a promising tool for conserving L. algarvense, but improvements are necessary for large-scale conservation efforts.eng
dc.description.sponsorship101052342; 101060874; CEECIND/00425/2017
dc.identifier.doi10.1007/s11240-025-03129-9
dc.identifier.eissn1573-5044
dc.identifier.issn0167-6857
dc.identifier.urihttp://hdl.handle.net/10400.1/28656
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relation.ispartofPlant Cell, Tissue and Organ Culture (PCTOC)
dc.rights.uriN/A
dc.subjectSea lavender
dc.subjectMicropropagation
dc.subjectConservation
dc.subjectPlant tissue culture
dc.subjectSalt-tolerant plant
dc.titleIn vitro propagation of Limonium algarvense Erben: towards the conservation of a rare and endemic halophyteeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUIDB/04326/2020
oaire.awardNumberUIDP/04326/2020
oaire.awardNumberLA/P/0101/2020
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.issue1
oaire.citation.titlePlant Cell, Tissue and Organ Culture
oaire.citation.volume162
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameCustódio
person.familyNameRodrigues
person.givenNameLuísa
person.givenNameMaria João
person.identifierR-004-VNG
person.identifier.ciencia-id791B-C560-AEA2
person.identifier.ciencia-id2514-0E17-1D8D
person.identifier.orcid0000-0003-4338-7703
person.identifier.orcid0000-0001-8732-710X
person.identifier.ridM-6101-2013
person.identifier.scopus-author-id15831018900
person.identifier.scopus-author-id56031608100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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