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Combined expression of p20 and p23 proteins from Citrus tristeza virus show enhanced local silencing suppressor activity

2016Journal article Open access
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dc.contributor.authorCosta, Angela A.
dc.contributor.authorMartins, Tatiana R.
dc.contributor.authorMarques, N T.
dc.contributor.authorNolasco, Gustavo
dc.contributor.editorFirenze University Press
dc.date.accessioned2017-04-07T15:57:31Z
dc.date.available2017-04-07T15:57:31Z
dc.date.issued2016
dc.description.abstractViruses developed a strategy to counter-defence the posttranscriptional gene silencing mechanism (PTGS) based on the activity of silencing suppressor proteins. Citrus tristeza virus (CTV), a member of the genus Closterovirus, has two suppressor proteins (p20 and p23) that target the local RNA silencing response of the host. In GFP transient co-expression assays performed on Nicotiana benthamiana 16C plants, local suppressor activity of p23 and p20 was similar. Co-expression of both proteins from a mild or a stem pitting CTV isolate showed stronger local suppression activity than either suppressor alone, with an increased GFP transcript level six-(for Gp M) to nine-fold (for Gp 3a) higher than non-inoculated 16C plants, in parallel with low accumulation of siRNAs. Further, GFP brightness of leaves infiltrated with Agrobacterium cultures at an OD600 of 0.5 was comparable to those infiltrated with OD600 0.25. These findings indicate that combined action of p20 and p23 proteins results in enhanced suppressor activity.
dc.identifier.doi10.14601/Phytopathol_Mediterr-16093
dc.identifier.issn0031-9465
dc.identifier.otherAUT: NMA00640; GNO00324;
dc.identifier.urihttp://hdl.handle.net/10400.1/9735
dc.language.isoeng
dc.peerreviewedyes
dc.relationCHARACTERIZATION OF PHYLOGENETICALLY DISTINCT SILENCING SUPPRESSORS OF CITRUS TRISTEZA VIRUS AND ASSESSMENT OF THE POSSIBILITY OF THEIR INHIBITION
dc.relation.isbasedonWOS:000381767900002
dc.titleCombined expression of p20 and p23 proteins from Citrus tristeza virus show enhanced local silencing suppressor activity
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCHARACTERIZATION OF PHYLOGENETICALLY DISTINCT SILENCING SUPPRESSORS OF CITRUS TRISTEZA VIRUS AND ASSESSMENT OF THE POSSIBILITY OF THEIR INHIBITION
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F62248%2F2009/PT
oaire.citation.endPage183
oaire.citation.issue2
oaire.citation.startPage172
oaire.citation.titlePhytopathologia Mediterranea
oaire.citation.volume55
person.familyNameTomás Marques
person.familyNameNolasco
person.givenNameNatália
person.givenNameGustavo
person.identifier.ciencia-id1510-CD2F-81CE
person.identifier.orcid0000-0002-0222-621X
person.identifier.orcid0000-0002-7025-6023
person.identifier.scopus-author-id6602625528
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication4b1751aa-958b-4d68-ae97-ea628673ba00
relation.isAuthorOfPublication87a8035f-bc15-44dd-b8c3-4288c5660fff
relation.isAuthorOfPublication.latestForDiscovery4b1751aa-958b-4d68-ae97-ea628673ba00
relation.isProjectOfPublication19079e03-f788-4fd0-a63b-67cc1545f2b2
relation.isProjectOfPublication.latestForDiscovery19079e03-f788-4fd0-a63b-67cc1545f2b2

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