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Molecular responses in the intestine of Atlantic Salmon (Salmo salar) following light and diet stimulation of smoltification: Potential molecular markers for a seawater-ready smolt

2025-02Journal article Open access
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dc.contributor.authorGaetano, Pasqualina
dc.contributor.authorFernandes Duarte, Vilma Vanessa
dc.contributor.authorStriberny, Anja
dc.contributor.authorHazlerigg, David G.
dc.contributor.authorJørgensen, Even H.
dc.contributor.authorCampinho, Marco António
dc.contributor.authorFuentes, Juan
dc.date.accessioned2024-12-10T13:43:26Z
dc.date.available2024-12-10T13:43:26Z
dc.date.issued2025-02
dc.description.abstractThe transfer to seawater (SW) represents a critical stage in the production of Atlantic salmon. The success of the transfer links with the optimal development of hypo-osmoregulatory capacities during smoltification. While various strategies are adopted in aquaculture to stimulate smoltification, considerable fish loss still occurs after transfer to sea cages. Therefore, we investigated the molecular responses in the anterior and posterior intestine of Atlantic salmon, following 1) a photoperiod treatment (24 h light (L):0 h dark (D) -* 24 L:0D vs. 7 L:17D -* 24 L:0D) and 2) dietary treatment (regular feed or feed enriched with a salt mix/tryptophan), combined with, or without a photoperiodic treatment in freshwater (FW), to evaluate how intestinal osmoregulatory mechanisms are modulated by these treatments, and to identify potential intestinal markers indicative of a SW-ready smolt. Using quantitative real-time PCR (qPCR), we investigated transcript levels of transporters and channels involved in ion movements through the enterocytes, tight junction components, and receptors (i.e., calcium-sensing receptor and prolactin receptor). The two intestinal regions showed different gene profiles and responsiveness towards the experimental treatments. In the anterior intestine, the exposure to short photoperiod (7 L:17D) upregulated Na+/K+ - ATPase subunit alpha 1c (nka alpha 1c), Na+/K+/2Cl- cotransporter 1 (nkcc1), Na+/K+/2Clcotransporter 2 (nkcc2), Cl- /HCO-3 exchanger Slc26a6 (slc26a6), and cystic fibrosis transmembrane conductance regulator I (cftrI), in FW and SW. Also, Na+/K+ - ATPase subunit alpha 1b (nka alpha 1b), occludin (ocln), and prolactin receptor (prlr) were upregulated in FW and claudin 15 (cldn15) in SW groups exposed to this photoperiod. The posterior intestine was less responsive to the experimental treatments, although upregulation of nkcc1, nkcc2, slc26a6, and cftrI was observed in FW in the short photoperiod groups. Hence, our findings show that exposure to a winter signal in FW more effectively activates hypo-osmoregulatory mechanisms in the intestine of Atlantic salmon, where a coordinated and complementary role of the anterior and posterior intestine ensures optimal SW processing. Dietary treatment had a positive but more marginal effect on the regulation of the genes investigated, mainly enhancing the impact of short photoperiod when the two treatments were combined. Overall, we propose the apical Na+/K+/2Cl- cotransporter, nkcc2, and the apical Cl- / HCO-3 exchanger, slc26a6, as potential FW molecular markers in the anterior intestine to assess "SW-readiness" in Atlantic salmon smolts.eng
dc.description.sponsorship901432;
dc.identifier.doi10.1016/j.aquaculture.2024.741742
dc.identifier.issn0044-8486
dc.identifier.urihttp://hdl.handle.net/10400.1/26443
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relationIntestinal physiology in Atlantic salmon: preparatory changes during smolting and after seawater entry
dc.relationThyroid in gut development during Atlantic salmon smoltification
dc.relationFunctional biology of thyroid hormones in teleost development
dc.relation.ispartofAquaculture
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAtlantic salmon
dc.subjectPhotoperiod
dc.subjectDietary treatment
dc.subjectIntestine
dc.subjectMolecular marker
dc.subjectHypo-osmoregulation
dc.titleMolecular responses in the intestine of Atlantic Salmon (Salmo salar) following light and diet stimulation of smoltification: Potential molecular markers for a seawater-ready smolteng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardTitleIntestinal physiology in Atlantic salmon: preparatory changes during smolting and after seawater entry
oaire.awardTitleThyroid in gut development during Atlantic salmon smoltification
oaire.awardTitleFunctional biology of thyroid hormones in teleost development
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F140045%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2021.04507.BD/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F01274%2F2014%2FCP1217%2FCT0007/PT
oaire.citation.startPage741742
oaire.citation.titleAquaculture
oaire.citation.volume596
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamInvestigador FCT
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameGaetano
person.familyNameFernandes Duarte
person.familyNameCampinho
person.givenNamePasqualina
person.givenNameVilma Vanessa
person.givenNameMarco António
person.identifier2193357
person.identifier.ciencia-idF110-5D6E-33CB
person.identifier.ciencia-id0E18-2560-6EC1
person.identifier.orcid0000-0001-5181-9073
person.identifier.orcid0000-0002-3007-6167
person.identifier.orcid0000-0002-5238-0506
person.identifier.ridD-8833-2013
person.identifier.scopus-author-id8938999600
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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