Repository logo
 
Publication

Evidence for the conservation of miR-223 in zebrafish (Danio rerio): implications for function

2015Journal article Restricted access
Simple item page
dc.contributor.authorRoberto, Vania Palma
dc.contributor.authorTiago, Daniel
dc.contributor.authorGautvik, K.
dc.contributor.authorCancela, M. Leonor
dc.date.accessioned2018-10-12T13:44:46Z
dc.date.available2018-10-12T13:44:46Z
dc.date.issued2015
dc.description.abstractMicroRNAs (miRNAs) are an abundant and conserved class of small RNAs, which play important regulatory functions by interacting with the 3' untranslated region (UTR) of target mRNAs. Through this mechanism, miR-223 was shown to regulate genes involved in mammalian haematopoiesis, both in physiological and pathological contexts. MiR-223 is essential for normal myelopoiesis in mammals, promoting granulocyte, osteoclast and megakaryocyte differentiation and suppressing erythropoiesis. However, there is a general lack of knowledge regarding miR-223 function in other vertebrates, which could help to clarify its role in other processes, such as development. In this work, we explored the functional conservation of miR-223 using zebrafish as a model. We show that miR-223 gene structure and genomic context have been maintained between human and zebrafish. In addition, we identified 22 novel sequences of miR-223 precursor and demonstrate that it contains domains highly conserved among vertebrates, suggesting function preservation throughout evolution. Furthermore, collected evidences show that miR-223 expression is highly correlated with haematopoietic events and osteoclastogenesis throughout zebrafish development. In adults, expression of miR-223 in zebrafish tissues mimics the distribution in mice, with high levels found in the major fish haematopoietic organ, the head kidney. These results suggest a conservation of miR-223 role in haematopoiesis, and osteoclastogenesis between zebrafish and human. Accordingly, validated targets of miR-223 in mammalian models were investigated and defined as putative targets in zebrafish, by in silico and gene expression analysis. Our data compiles critical evidence showing that miR-223, a highly conserved miRNA, appears to have kept similar regulatory functions throughout evolution.pt_PT
dc.description.sponsorshipThis work was supported by a prize granted by the Calouste Gulbenkian Foundation (program “Na Fronteira das Ciências da Vida - 2009”; to D.M.T.), by the European Regional Development Fund (ERDF) through COMPETE Program and by national funds through the FCT — Foundation for Science and Technology, under the project “PEst-C/MAR/LA0015/ 2011” and by Helse SørØst, Norway. V.P.R. and D.M.T. were the recipients of doctoral (SFRH/BD/38607/ 2007) and post-doctoral (SFRH/BPD/45034/2008) fellowships respectively, fromthe Portuguese Foundation for Science and Technology (FCT)pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.gene.2015.04.022pt_PT
dc.identifier.issn0378-1119
dc.identifier.urihttp://hdl.handle.net/10400.1/10868
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.hasversionhttps://sapientia.ualg.pt/handle/10400.1/9660
dc.subjectAnimalspt_PT
dc.subjectCloningpt_PT
dc.subjectEvolutionpt_PT
dc.subjectHematopoiesispt_PT
dc.subjectHumanspt_PT
dc.subjectMammalspt_PT
dc.subjectMicroRNAspt_PT
dc.subjectOsteoclastspt_PT
dc.subjectPhylogenypt_PT
dc.subjectZebrafishpt_PT
dc.subjectMolecularpt_PT
dc.titleEvidence for the conservation of miR-223 in zebrafish (Danio rerio): implications for functionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F45034%2F2008/PT
oaire.citation.endPage62pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage54pt_PT
oaire.citation.titleGenept_PT
oaire.citation.volume566pt_PT
oaire.fundingStreamSFRH
person.familyNameRoberto
person.familyNameTiago
person.familyNameCancela
person.givenNameVânia
person.givenNameDaniel
person.givenNameM. Leonor
person.identifier1230353
person.identifier.ciencia-id681E-40B7-331F
person.identifier.orcid0000-0002-8134-4045
person.identifier.orcid0000-0001-8418-6292
person.identifier.orcid0000-0003-3114-6662
person.identifier.scopus-author-id27467657800
person.identifier.scopus-author-id14422711000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationc730a868-0e9f-43e4-a354-10130b30b764
relation.isAuthorOfPublication10cda3a0-7b9c-4d16-bd2a-a06df1d56d94
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublication.latestForDiscoveryb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isProjectOfPublication0ca5dd9c-36ef-4d34-a832-0b946816c8c6
relation.isProjectOfPublication.latestForDiscovery0ca5dd9c-36ef-4d34-a832-0b946816c8c6

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Roberto-2015-Evidence-for-the-conservation-of-mi.pdf
Size:
1.33 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.41 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)