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Water absorption and bicarbonate secretion in the intestine of the sea bream are regulated by transmembrane and soluble adenylyl cyclase stimulation

2012Journal article Restricted access
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dc.contributor.authorCarvalho, Edison Samir Mascarelhas
dc.contributor.authorGregorio, Silvia
dc.contributor.authorPower, Deborah
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorFuentes, J.
dc.date.accessioned2014-05-09T14:04:01Z
dc.date.available2014-05-09T14:04:01Z
dc.date.issued2012
dc.date.updated2014-05-08T09:52:42Z
dc.description.abstractIn the marine fish intestine luminal, HCO3 - can remove divalent ions (calcium and magnesium) by precipitation in the form of carbonate aggregates. The process of epithelial HCO3 - secretion is under endocrine control, therefore, in this study we aimed to characterize the involvement of transmembrane (tmACs) and soluble (sACs) adenylyl cyclases on the regulation of bicarbonate secretion (BCS) and water absorption in the intestine of the sea bream (Sparus aurata). We observed that all sections of sea bream intestine are able to secrete bicarbonate as measured by pH–Stat in Ussing chambers. In addition, gut sac preparations reveal net water absorption in all segments of the intestine, with significantly higher absorption rates in the anterior intestine that in the rectum. BCS and water absorption are positively correlated in all regions of the sea bream intestinal tract. Furthermore, stimulation of tmACs (10 lM FK? 500 lM IBMX) causes a significant decrease in BCS, bulk water absorption and short circuit current (Isc) in a region dependent manner. In turn, stimulation of sACs with elevated HCO3 - results in a significant increase in BCS, and bulk water absorption in the anterior intestine, an action completely reversed by the sAC inhibitor KH7 (200 lM). Overall, the results reveal a functional relationship between BCS and water absorption in marine fish intestine and modulation by tmACs and sAC. In light of the present observations, it is hypothesized that the endocrine effects on intestinal BCS and water absorption mediated by tmACs are locally and reciprocally modulated by the action of sACs in the fish enterocyte, thus fine-tuning the process of carbonate aggregate production in the intestinal lumen.por
dc.identifier.citationCarvalho, Edison S. M.; Gregório, Sílvia F.; Power, Deborah M.; Canário, Adelino V. M.; Fuentes, Juan. Water absorption and bicarbonate secretion in the intestine of the sea bream are regulated by transmembrane and soluble adenylyl cyclase stimulation, Journal of Comparative Physiology B, 182, 8, 1069-10, 2012.por
dc.identifier.doihttp://dx.doi.org/10.1007/s00360-012-0685-4
dc.identifier.issn0174-1578
dc.identifier.otherAUT: DPO00386; ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3830
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.subjectBicarbonate secretionpor
dc.subjectIntestinal tractpor
dc.subjectMarine fishpor
dc.subjectpH–Statpor
dc.subjectSoluble adenylyl cyclasepor
dc.subjectcAMPpor
dc.subjectTransmembrane adenylyl cyclasepor
dc.subjectUssing chamberpor
dc.subjectWater absorptionpor
dc.titleWater absorption and bicarbonate secretion in the intestine of the sea bream are regulated by transmembrane and soluble adenylyl cyclase stimulationpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1080por
oaire.citation.issue8por
oaire.citation.startPage1069por
oaire.citation.titleJournal of Comparative Physiology Bpor
oaire.citation.volume182por
person.familyNameGregorio
person.familyNamePower
person.familyNameCanario
person.familyNameFuentes
person.givenNameSilvia
person.givenNameDeborah Mary
person.givenNameAdelino
person.givenNameJuan
person.identifier1579837
person.identifier143624
person.identifier392998
person.identifier.ciencia-idBF14-020B-4C39
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id421B-E196-2C33
person.identifier.orcid0000-0002-6648-5122
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-0430-8734
person.identifier.ridG-1618-2018
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7201832526
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublicationd354b937-b18c-4ca5-a843-04a04269d60f
relation.isAuthorOfPublication.latestForDiscoveryd354b937-b18c-4ca5-a843-04a04269d60f

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