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Effects of preslaughter stress levels on the post-mortem sarcoplasmic proteomic profile of gilthead seabream muscle

2012Journal article Restricted access
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dc.contributor.authorSilva, Tomé S.
dc.contributor.authorCordeiro, O.
dc.contributor.authorMatos, Elisabete
dc.contributor.authorWulff, Tune
dc.contributor.authorDias, J.
dc.contributor.authorJessen, Flemming
dc.contributor.authorRodrigues, Pedro
dc.date.accessioned2014-06-18T10:03:19Z
dc.date.available2014-06-18T10:03:19Z
dc.date.issued2012
dc.date.updated2014-06-12T11:30:33Z
dc.description.abstractFish welfare is an important concern in aquaculture, not only due to the ethical implications but also for productivity and quality-related reasons. The purpose of this study was to track soluble proteome expression in post-mortem gilthead seabream muscle and to obsere how preslaughter stress affects these post-mortem processes. For the experiment, two groups of gilthead seabream (n = 5) were subjected to distinct levels of preslaughter stress, with three muscle samples being taken from each fish. Proteins were extracted from the muscle samples, fractionated, and separated by 2DE. Protein identification was performed by MALDI-TOF-TOF MS. Analysis of the results indicates changes on several cellular pathways, with some of these changes being attributable to oxidative and proteolytic activity on sarcoplasmic proteins, together with leaking of myofibrillar proteins. These processes appear to have been hastened by preslaughter stress, confirming that it induces clear postmortem changes in the muscle proteome of gilthead seabream.por
dc.identifier.citationSilva, Tomé S.; Cordeiro, Odete D.; Matos, Elisabete D.; Wulff, Tune; Dias, Jorge P.; Jessen, Flemming; Rodrigues, Pedro M. Effects of Preslaughter Stress Levels on the Post-mortem Sarcoplasmic Proteomic Profile of Gilthead Seabream Muscle, Journal of Agricultural and Food Chemistry, 60, 37, 9443-9453, 2012.por
dc.identifier.doihttp://dx.doi.org/10.1021/jf301766e |
dc.identifier.issn0021-8561
dc.identifier.otherAUT: PMR01268;
dc.identifier.urihttp://hdl.handle.net/10400.1/4363
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAmerican Chemical Societypor
dc.subjectGilthead seabreampor
dc.subjectPreslaughter stresspor
dc.subjectProteomicspor
dc.subjectPost-mortem musclepor
dc.subjectSarcoplasmic proteinspor
dc.titleEffects of preslaughter stress levels on the post-mortem sarcoplasmic proteomic profile of gilthead seabream musclepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage9453por
oaire.citation.issue37por
oaire.citation.startPage9443por
oaire.citation.titleJournal of Agricultural and Food Chemistrypor
oaire.citation.volume60por
person.familyNameRodrigues
person.givenNamePedro
person.identifier.orcid0000-0002-9668-1204
person.identifier.ridM-3406-2013
person.identifier.scopus-author-id55107531000
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationc4613fe6-e1a0-4dd9-be8d-442b2b8a7b72
relation.isAuthorOfPublication.latestForDiscoveryc4613fe6-e1a0-4dd9-be8d-442b2b8a7b72

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