dc.contributor.advisor	Barros, Raúl José Jorge de
dc.contributor.advisor	Grey, Carl
dc.contributor.author	Almeida, Dulce
dc.date.accessioned	2013-10-30T15:32:28Z
dc.date.available	2013-10-30T15:32:28Z
dc.date.issued	2011
dc.description	Dissertação de mest., Engenharia Biológica, Faculdade de Ciências e Tecnologia, Univ. do Algarve, 2011	por
dc.description.abstract	The purpose of this work is to investigate and improve different technical aspects in the extraction of soluble dietary fibres, mostly AX, from rye bran and to characterize the fibres throughout the process, as well as perform enzymatic hydrolysis of arabinoxylans, producing health promoting oligosaccharides: Xylan-oligosaccharides and arabinoxylan-oligosaccharides. These hydrolysis products may serve as candidate supplements in food and feed industry because of their prebiotic proprieties. They can act as substrates which selectively stimulate the growth of probiotic bacteria that produce metabolic active compounds which can contribute, for instance, to lowering risk of colon cancer incidence, decrease cholesterol absorption and might have a positive influence on type II diabetes and obesity. The work included several enzymatic steps, using alpha-amylases, proteases and xylanses as well as extraction with steam, separation steps and purification using solvent precipitation, dialysis and freeze drying. Different analytical techniques were used to follow the enzymatic steps and to characterize the carbohydrates, including an OPA-based spectrophotometrical method to study the protein hydrolysis, acid hydrolysis in combination with High Performance Anion Exchange Chromatography with Pulsed Ampherometric Detection (HPAEC-PAD) and size-exclusion chromatography (SEC) to study the carbohydrates. As main results, regarding the extraction final product, a visible improvement in is physical appearance is achieved, as well as a significant improvement in its content in arabinoxylans which increases from 37.57% (using the previous method) up to 60.45%. Regarding the arabinoxylan / glucose ratio, it also improved from 1,84 up to a maximum of 10.02. The objective of this work was achieved with success as a significant improvement on the product composition and characteristics was achieved as well as the heath beneficial oligosaccharides were obtained, therefore, this work result on a small but good contribution for the project of my colleagues in Lund University.	por
dc.identifier.uri	http://hdl.handle.net/10400.1/3116
dc.language.iso	eng	por
dc.peerreviewed	yes	por
dc.subject	Alimentos funcionais	por
dc.subject	Arabinoxilanos	por
dc.subject	Xilanase	por
dc.subject	Prebióticos	por
dc.subject	Probióticos	por
dc.subject	Functional foods	por
dc.subject	Arabinoxylan	por
dc.subject	Xylanase	por
dc.subject	Prebiotics	por
dc.subject	Probiotics	por
dc.title	Improved extraction of arabinoxylans from rye bran and production of health beneficial oligosaccharides	por
dc.type	master thesis
dspace.entity.type	Publication
person.familyName	Almeida
person.givenName	Dulce
person.identifier.orcid	0000-0002-1399-4062
rcaap.rights	openAccess	por
rcaap.type	masterThesis	por
relation.isAuthorOfPublication	5704a415-76d8-4124-ad26-dfa8efb6739b
relation.isAuthorOfPublication.latestForDiscovery	5704a415-76d8-4124-ad26-dfa8efb6739b
thesis.degree.grantor	Universidade do Algarve. Faculdade de Ciências e Tecnologia	por
thesis.degree.level	Mestre	por
thesis.degree.name	Mestrado em Engenharia Biológica	por

Improved extraction of arabinoxylans from rye bran and production of health beneficial oligosaccharides

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