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The freshwater Sponge Ephydatia Fluviatilis harbours diverse pseudomonas species (Gammaproteobacteria, Pseudomonadales) with broad-spectrum antimicrobial activity

2014-02Journal article Open access
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dc.contributor.authorKeller-Costa, Tina
dc.contributor.authorJousset, Alexandre
dc.contributor.authorvan Overbeek, Leo
dc.contributor.authorvan Elsas, Jan Dirk
dc.contributor.authorCosta, Rodrigo
dc.date.accessioned2018-12-07T14:53:00Z
dc.date.available2018-12-07T14:53:00Z
dc.date.issued2014-02
dc.description.abstractBacteria are believed to play an important role in the fitness and biochemistry of sponges (Porifera). Pseudomonas species (Gammaproteobacteria, Pseudomonadales) are capable of colonizing a broad range of eukaryotic hosts, but knowledge of their diversity and function in freshwater invertebrates is rudimentary. We assessed the diversity, structure and antimicrobial activities of Pseudomonas spp. in the freshwater sponge Ephydatia fluviatilis. Polymerase Chain Reaction - Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprints of the global regulator gene gacA revealed distinct structures between sponge-associated and free-living Pseudomonas communities, unveiling previously unsuspected diversity of these assemblages in freshwater. Community structures varied across E. fluviatilis specimens, yet specific gacA phylotypes could be detected by PCR-DGGE in almost all sponge individuals sampled over two consecutive years. By means of whole-genome fingerprinting, 39 distinct genotypes were found within 90 fluorescent Pseudomonas isolates retrieved from E. fluviatilis. High frequency of in vitro antibacterial (49%), antiprotozoan (35%) and anti-oomycetal (32%) activities was found among these isolates, contrasting less-pronounced basidiomycetal (17%) and ascomycetal (8%) antagonism. Culture extracts of highly predation-resistant isolates rapidly caused complete immobility or lysis of cells of the protozoan Colpoda steinii. Isolates tentatively identified as P. jessenii, P. protegens and P. oryzihabitans showed conspicuous inhibitory traits and correspondence with dominant sponge-associated phylotypes registered by cultivation-independent analysis. Our findings suggest that E. fluviatilis hosts both transient and persistent Pseudomonas symbionts displaying antimicrobial activities of potential ecological and biotechnological value.
dc.description.sponsorshipEuropean Regional Development Fund (ERDF) through the COMPETE (Operational Competitiveness Programme); national funds through FCT (Foundation for Science and Technology) [PEst-C/MAR/LA0015/2011]; FCT-funded project [PTDC/BIA-MIC/3865/2012]; Federation of European Microbiological Societies (FEMS)
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1371/journal.pone.0088429
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.1/11310
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPublic Library of Science
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectFluorescent pseudomonads
dc.subjectSecondary metabolites
dc.subjectPhenotypic variation
dc.subjectBacterial symbiont
dc.subjectBiological-control
dc.subjectBiofilm formation
dc.subjectSmall Rnas
dc.subjectSp Nov.
dc.subjectRhizosphere
dc.subjectCommunity
dc.titleThe freshwater Sponge Ephydatia Fluviatilis harbours diverse pseudomonas species (Gammaproteobacteria, Pseudomonadales) with broad-spectrum antimicrobial activity
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIA-MIC%2F3865%2F2012/PT
oaire.citation.issue2
oaire.citation.startPagee88429
oaire.citation.titlePLoS ONE
oaire.citation.volume9
oaire.fundingStream3599-PPCDT
person.familyNameKeller-Costa
person.familyNameda Silva Costa
person.givenNameTina
person.givenNameRodrigo
person.identifier115920
person.identifier.ciencia-id9319-A7D5-5335
person.identifier.ciencia-id5917-D500-D251
person.identifier.orcid0000-0003-3702-9192
person.identifier.orcid0000-0002-5932-4101
person.identifier.ridN-7274-2013
person.identifier.scopus-author-id7203063627
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublicatione5a30366-3797-42ff-944b-f29ba4136cbd
relation.isAuthorOfPublication4495127c-16f2-4231-9fc1-b471c661036b
relation.isAuthorOfPublication.latestForDiscoverye5a30366-3797-42ff-944b-f29ba4136cbd
relation.isProjectOfPublication6d45a925-1c5f-4d4e-bc89-1b8f382b36d4
relation.isProjectOfPublication.latestForDiscovery6d45a925-1c5f-4d4e-bc89-1b8f382b36d4

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