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In vitro and in silico approaches to appraise Polygonum maritimum L. as a source of innovative products with anti-ageing potential

2018-01Journal article Restricted access
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dc.contributor.authorRodrigues, Maria Joao
dc.contributor.authorSlusarczyk, Sylwester
dc.contributor.authorPecio, Łukasz
dc.contributor.authorMatkowski, Adam
dc.contributor.authorSalmas, Ramin Ekhteiari
dc.contributor.authorDurdagi, Serdar
dc.contributor.authorPereira, Catarina
dc.contributor.authorVarela, J.
dc.contributor.authorBarreira, Luísa
dc.contributor.authorCustódio, Luísa
dc.date.accessioned2018-02-05T15:02:47Z
dc.date.available2020-01-01T01:30:11Z
dc.date.issued2018-01
dc.description.abstractDifferent Polygonum species have in vitro neuroprotective properties and are traditionally used for their anti-ageing benefits. In this context this work explored for the first time P. maritimum (sea knotgrass) as a potential source of natural products with industrial application as cognitive enhancers with anti-ageing potential. For that purpose methanol and dichloromethane extracts were prepared from leaves and roots of that species and evaluated for in vitro neuroprotective effects through the inhibition on acetyl- (AChE) and butyrylcholinesterase (BuChE), protection against hydrogen peroxide (H2O2)-induced cytoxicity on neuroblastoma cells and lipopolysaccharide (LPS)-induced neuroinflammation on microglia cells. The extracts were chemically characterized by ultra-high-pressure liquid chromatography (UHPLC) coupled to tandem mass spectrometry (MS/MS) and docking studies were performed on the identified compounds. Methanol extracts had the highest activity in AChE (leaves: IC50 = 0.27 mg/mL; roots: IC50 = 0.17 mg/mL) and BuChE (leaves: IC50 = 0.62 mg/mL; roots: IC50 = 0.61 mg/mL) inhibition, as well as reduction of nitric oxide (NO) production in LPS-treated microglia (leaves: IC50 = 4.17 μg/mL; roots: IC50 = 9.95 μg/mL). Methanol extracts prevented oxidative stress-induced cytotoxicity in SH-SY5Y cells when applied simultaneously with H2O2, whereas cells pre-treated with the dichloromethane extracts had increased viability. Fifteen flavonoids were identified and showed favorable binding energies to AChE and BuChE binding pockets. These data suggests P. maritimum as a promising source of natural products and/or molecules with cognitive enhancement and anti-ageing properties.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.indcrop.2017.10.046pt_PT
dc.identifier.issn0926-6690
dc.identifier.otherAUT: LBA00541; JVA01305;
dc.identifier.urihttp://hdl.handle.net/10400.1/10358
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCentre of Marine Sciences
dc.relationUnravelling the biotechnological potential of halophyte species from the Algarve coast - HaloBiotech
dc.relationBiologically Active Natural Products from Marine Organisms and Salt Marsh Plants (acronym: PharmaBlue)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectHalophytespt_PT
dc.subjectCholinesterase inhibitionpt_PT
dc.subjectOxidative stresspt_PT
dc.subjectMolecular docking simulationspt_PT
dc.subjectNeuroinflammationpt_PT
dc.subjectMolecular modelingpt_PT
dc.subjectMolecular dynamics simulationspt_PT
dc.titleIn vitro and in silico approaches to appraise Polygonum maritimum L. as a source of innovative products with anti-ageing potentialpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre of Marine Sciences
oaire.awardTitleUnravelling the biotechnological potential of halophyte species from the Algarve coast - HaloBiotech
oaire.awardTitleBiologically Active Natural Products from Marine Organisms and Salt Marsh Plants (acronym: PharmaBlue)
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F94407%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F116604%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00049%2F2012%2FCP0179%2FCT0001/PT
oaire.citation.endPage399pt_PT
oaire.citation.startPage391pt_PT
oaire.citation.titleIndustrial Crops and Productspt_PT
oaire.citation.volume111pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamSFRH
oaire.fundingStreamOE
oaire.fundingStreamInvestigador FCT
person.familyNameRodrigues
person.familyNameGuerreiro Pereira
person.familyNameVarela
person.familyNameBarreira
person.familyNameCustódio
person.givenNameMaria João
person.givenNameCatarina Alexandra
person.givenNameJoão
person.givenNameLuísa
person.givenNameLuísa
person.identifierR-004-VNG
person.identifierNmxO5SIAAAAJ
person.identifier.ciencia-id2514-0E17-1D8D
person.identifier.ciencia-id4512-3435-689C
person.identifier.ciencia-id6D1A-17E5-1400
person.identifier.ciencia-id791B-C560-AEA2
person.identifier.orcid0000-0001-8732-710X
person.identifier.orcid0000-0002-1131-8773
person.identifier.orcid0000-0003-3101-693X
person.identifier.orcid0000-0002-4077-855X
person.identifier.orcid0000-0003-4338-7703
person.identifier.ridM-4223-2013
person.identifier.ridM-6101-2013
person.identifier.scopus-author-id56031608100
person.identifier.scopus-author-id55741416000
person.identifier.scopus-author-id15831018900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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