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Chlorophyll a Fluorescence: a Fast and Low-Cost Tool to Detect Superficial Scald in 'Rocha' Pear (Pyrus communis L. 'Rocha')?

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dc.contributor.authorGarde, I.
dc.contributor.authorAntunes, Maria Dulce
dc.contributor.authorda Silva, J. M.
dc.contributor.authorGuerra, Rui Manuel Farinha das Neves
dc.contributor.authorCavaco, A. M.
dc.date.accessioned2015-06-19T13:47:31Z
dc.date.available2015-06-19T13:47:31Z
dc.date.issued2012
dc.description.abstractThis study aimed to test whether the chlorophyll a (Chla) fluorescence determined by a low-cost non-modulated fluorometer could provide fast, reliable and non-invasive estimators of superficial scald in 'Rocha' pear (Pyrus communis L. 'Rocha'). Fruit were harvested before the optimal maturation stage and cold stored under normal atmosphere for 7 months (NA: 0 degrees C, 90-95% RH) and 2 in controlled atmosphere (CA: 0 degrees C, 90-95% RH, 1.5 kPaO(2) + 0.5 kPa CO2) (T), or harvested at the optimal maturation stage and cold stored for 9 months under CA (C). Then, they were transferred to shelf-life conditions (22+/-2 degrees C, 70% RH) and followed for 7 d. Chla fluorescence, scald index (SI), ripening attributes, alpha-farnesene, conjugated trienols, and photosynthetic pigments were determined for each pear in both groups. Conditions chosen before shelf-life did not prevent the subsequent ripening of any fruit, but changed dramatically the superficial scald development pattern: in C fruit, the disorder developed progressively during shelf-life, whereas in T fruit, it peaked during storage. C fruit exhibited a significant negative correlation (R=-0.65; p<0.05) between Fv/Fm and scald development, but not with ripening (R=-0.15; p<0.05). As expected, the opposite was observed in T fruit, in which only a low, positive, yet significant correlation was found between Fv/Fm and ripening (R=0.44; p<0.05). The multiple regression approach using Fv/Fm and other Chla fluorescence parameters produced an equation from which we calculated the 'predicted' scald index in C fruit. This correlated clearly (R=0.73; p<0.05) with the real values visually assessed. If color values a*, b* and Hue were included in this multiple regression, the correlation was significantly enhanced (0.91; p<0.05). Although preliminary, this study has shown that basic Chla fluorescence parameters are valuable estimators of superficial scald in 'Rocha' pear and might be used in the early detection of the disorder.
dc.identifier.isbn978-90-66053-78-6
dc.identifier.issn0567-7572
dc.identifier.otherAUT: MAN00114;
dc.identifier.urihttp://hdl.handle.net/10400.1/6469
dc.language.isoeng
dc.peerreviewedyes
dc.publisherInt. Soc. Horticultural Science
dc.relation.isbasedonP-002-E2X
dc.relation.ispartofActa Horticulturae
dc.titleChlorophyll a Fluorescence: a Fast and Low-Cost Tool to Detect Superficial Scald in 'Rocha' Pear (Pyrus communis L. 'Rocha')?
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceLisbon, Portugal
oaire.citation.endPage166
oaire.citation.startPage159
oaire.citation.title28th International Horticultural Congress on Science and Horticulture for People (IHC) / International Symposium on Postharvest Technology in the Global Market
oaire.citation.volume934
person.familyNameAntunes
person.familyNameGuerra
person.familyNameCavaco Guerra
person.givenNameMaria Dulce
person.givenNameRui
person.givenNameAna Margarida
person.identifier177556
person.identifierC-1285-2012
person.identifier.ciencia-idC11B-9B05-217E
person.identifier.ciencia-id3D16-5067-D6BB
person.identifier.ciencia-idC91E-B434-E327
person.identifier.orcid0000-0002-8913-6136
person.identifier.orcid0000-0002-8642-5792
person.identifier.orcid0000-0003-2708-5991
person.identifier.ridA-4683-2012
person.identifier.scopus-author-id7102645075
person.identifier.scopus-author-id6602899707
rcaap.rightsopenAccess
rcaap.typeconferenceObject
relation.isAuthorOfPublication7947cc50-4ae0-4ada-8ddf-081f247adc90
relation.isAuthorOfPublicationeff7071e-e676-465b-bae3-983f522acd98
relation.isAuthorOfPublication18ad736f-b1f3-4a19-9636-e7a80248cf94
relation.isAuthorOfPublication.latestForDiscoveryeff7071e-e676-465b-bae3-983f522acd98

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