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Osmostress-induced changes in yeast gene expression
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Orientador(es)
Resumo(s)
When Saccharomyces cerevisiae cells are exposed to high concentrations of NaCI, they show reduced viability, methionine uptake and protein biosynthesis. Cells can acquire tolerance against a severe salt shock (up to 1.4 M NaCI) by a previous treatment with 0.7 M NaCI, but not by a previous heat shock. Two-dimensional analysis of [3H]-leucine-labelled proteins from salt-shocked cells (0.7 M NaCt) revealed the elevated rate of synthesis of nine proteins, among which were the heat-shock proteins hsp12 and hsp26. Northern analysis using gene-specific probes confirmed the identity of the latter proteins and, in addition, demonstrated the induction of glycerol-3-phos-phate dehydrogenase gene expression. The synthesis of the same set of proteins is induced or enhanced upon exposure of cells to 0.8 M sucrose, although not as dramatically as in an iso-osmolar NaCI concentration (0.7 M).
Descrição
Palavras-chave
Yeast Osmotic stress Salt stress Heat shock proteins
Contexto Educativo
Citação
Varela, J.C., van Beekvelt, C., Planta, R.J. & Mager, W.H. (1992). Osmostress-induced changes in yeast gene expression. Molecular Microbiology, 6: 2183-2190.
Editora
Wiley