Osmostress-induced changes in yeast gene expression

Varela, J.; Van Beekvelt, Catelijne; Planta, Rudi; Mager, Peter

http://hdl.handle.net/10400.1/4831

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Authors

Varela, J.

Van Beekvelt, Catelijne

Planta, Rudi

Mager, Peter

Abstract(s)

When Saccharomyces cerevisiae cells are exposed to high concentrations of NaCI, they show reduced viability, methionine uptake and protein biosynthesis. Cells can acquire tolerance against a severe salt shock (up to 1.4 M NaCI) by a previous treatment with 0.7 M NaCI, but not by a previous heat shock. Two-dimensional analysis of [3H]-leucine-labelled proteins from salt-shocked cells (0.7 M NaCt) revealed the elevated rate of synthesis of nine proteins, among which were the heat-shock proteins hsp12 and hsp26. Northern analysis using gene-specific probes confirmed the identity of the latter proteins and, in addition, demonstrated the induction of glycerol-3-phos-phate dehydrogenase gene expression. The synthesis of the same set of proteins is induced or enhanced upon exposure of cells to 0.8 M sucrose, although not as dramatically as in an iso-osmolar NaCI concentration (0.7 M).

Keywords

Yeast Osmotic stress Salt stress Heat shock proteins

URI

http://hdl.handle.net/10400.1/4831

Citation

Varela, J.C., van Beekvelt, C., Planta, R.J. & Mager, W.H. (1992). Osmostress-induced changes in yeast gene expression. Molecular Microbiology, 6: 2183-2190.