Publication
Osmostress-induced changes in yeast gene expression
| dc.contributor.author | Varela, J. | |
| dc.contributor.author | Van Beekvelt, Catelijne | |
| dc.contributor.author | Planta, Rudi | |
| dc.contributor.author | Mager, Peter | |
| dc.date.accessioned | 2014-07-23T16:00:23Z | |
| dc.date.available | 2014-07-23T16:00:23Z | |
| dc.date.issued | 1992 | |
| dc.description.abstract | When Saccharomyces cerevisiae cells are exposed to high concentrations of NaCI, they show reduced viability, methionine uptake and protein biosynthesis. Cells can acquire tolerance against a severe salt shock (up to 1.4 M NaCI) by a previous treatment with 0.7 M NaCI, but not by a previous heat shock. Two-dimensional analysis of [3H]-leucine-labelled proteins from salt-shocked cells (0.7 M NaCt) revealed the elevated rate of synthesis of nine proteins, among which were the heat-shock proteins hsp12 and hsp26. Northern analysis using gene-specific probes confirmed the identity of the latter proteins and, in addition, demonstrated the induction of glycerol-3-phos-phate dehydrogenase gene expression. The synthesis of the same set of proteins is induced or enhanced upon exposure of cells to 0.8 M sucrose, although not as dramatically as in an iso-osmolar NaCI concentration (0.7 M). | por |
| dc.description.sponsorship | FCT (formerly known as JNICT). | por |
| dc.identifier.citation | Varela, J.C., van Beekvelt, C., Planta, R.J. & Mager, W.H. (1992). Osmostress-induced changes in yeast gene expression. Molecular Microbiology, 6: 2183-2190. | por |
| dc.identifier.doi | http://dx.doi.org/10.1111/j.1365-2958.1992.tb01392.x | |
| dc.identifier.other | AUT: JVA01305 | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/4831 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Wiley | por |
| dc.subject | Yeast | por |
| dc.subject | Osmotic stress | por |
| dc.subject | Salt stress | por |
| dc.subject | Heat shock proteins | por |
| dc.title | Osmostress-induced changes in yeast gene expression | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 2190 | por |
| oaire.citation.issue | 15 | por |
| oaire.citation.startPage | 2183 | por |
| oaire.citation.volume | 6 | por |
| person.familyName | Varela | |
| person.givenName | João | |
| person.identifier.orcid | 0000-0003-3101-693X | |
| person.identifier.rid | M-4223-2013 | |
| person.identifier.scopus-author-id | 55741416000 | |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |
| relation.isAuthorOfPublication | d339a9e7-bc32-49c4-ba48-a17343d40092 | |
| relation.isAuthorOfPublication.latestForDiscovery | d339a9e7-bc32-49c4-ba48-a17343d40092 |