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Yeast β-glucans and microalgal extracts modulate the immune response and gut microbiome in Senegalese sole (Solea senegalensis)

2019Journal article Restricted access
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dc.contributor.authorCarballo, Carlos
dc.contributor.authorPinto, Patricia IS
dc.contributor.authorMateus, Ana
dc.contributor.authorBerbel, Concha
dc.contributor.authorGuerreiro, Claudia
dc.contributor.authorMartinez-Blanch, Juan F.
dc.contributor.authorCodoñer, Francisco M.
dc.contributor.authorMantecon, Lalia
dc.contributor.authorPower, Deborah
dc.contributor.authorManchado, Manuel
dc.date.accessioned2020-03-23T10:17:17Z
dc.date.available2020-09-01T00:30:13Z
dc.date.issued2019
dc.description.abstractOne bottleneck to sustainability of fish aquaculture is the control of infectious diseases. Current trends include the preventive application of immunostimulants and prebiotics such as polysaccharides. The present study investigated how yeast β-glucan (Y), microalgal polysaccharide-enriched extracts (MAe) and whole Phaeodactylum tricornutum cells (MA) modulated the gut microbiome and stimulated the immune system in Senegalese sole (Solea senegalensis) when administered by oral intubation. Blood, intestine and spleen samples were taken at 3 h, 24 h, 48 h and 7 days after treatment. The short-term response (within 48 h after treatment) consisted of up-regulation of il1b and irf7 expression in the gut of the Y treated group. In contrast, administration of MAe decreased expression of tnfa and the chemokine cxc10 in the gut and spleen. Both treatments down-regulated the expression of irf3 with respect to the control group. Lysozyme activity in plasma decreased at 48 h only in the MAe-treated soles. Medium-term response consisted of the up-regulation of clec and irf7 expression in the gut of the Y, MAe and MA groups and of il1b mRNAs in the spleen of the MA group compared to the control group. Microbiome analysis using 16S rDNA gene sequencing indicated that the intestine microbiome was dominated by bacteria of the Vibrio genus (>95%). All the treatments decreased the relative proportion of Vibrio in the microbiome and Y and MAe decreased and MA increased diversity. Quantitative PCR confirmed the load of bacteria of the Vibrio genus was significantly decreased and this was most pronounced in Y treated fish. These data indicate that orally administrated insoluble yeast β-glucans acted locally in the gut modulating the immune response and controlling the Vibrio abundance. In contrast, the MAe slightly reduced the Vibrio load in the intestine and caused a transient systemic anti-inflammatory response. The results indicate that these polysaccharides are a promising source of prebiotics for the sole aquaculture industry.pt_PT
dc.description.sponsorshipH2020 MSCA-RISE project 691102 CCMAR/Multi/04326/2016pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.fsi.2019.05.044pt_PT
dc.identifier.issn1050-4648
dc.identifier.urihttp://hdl.handle.net/10400.1/13637
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationDevelopment of Microalgae-based novel high added-value products for the Cosmetic and Aquaculture industry
dc.subjectAnimal Feedpt_PT
dc.subjectAnimalspt_PT
dc.subjectDiatomspt_PT
dc.subjectDietpt_PT
dc.subjectFlatfishespt_PT
dc.subjectGastrointestinal microbiomept_PT
dc.subjectImmunitypt_PT
dc.subjectMicroalgaept_PT
dc.subjectPrebioticspt_PT
dc.subjectRandom allocationpt_PT
dc.subjectYeastpt_PT
dc.subjectBeta-Glucanspt_PT
dc.titleYeast β-glucans and microalgal extracts modulate the immune response and gut microbiome in Senegalese sole (Solea senegalensis)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDevelopment of Microalgae-based novel high added-value products for the Cosmetic and Aquaculture industry
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F84033%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/691102/EU
oaire.citation.endPage39pt_PT
oaire.citation.startPage31pt_PT
oaire.citation.titleFish and Shellfish Immunologypt_PT
oaire.citation.volume92pt_PT
oaire.fundingStreamSFRH
oaire.fundingStreamH2020
person.familyNamePinto
person.familyNameMateus
person.familyNameGuerreiro
person.familyNamePower
person.givenNamePatricia IS
person.givenNameAna Patrícia
person.givenNameClaudia
person.givenNameDeborah Mary
person.identifier643457
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.ciencia-id2816-E389-626E
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-7854-3898
person.identifier.orcid0000-0003-2045-1047
person.identifier.orcid0000-0003-3585-9563
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-3817-2013
person.identifier.scopus-author-id10240774300
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication64bc526e-5281-44e5-91eb-5743436109f9
relation.isAuthorOfPublication74cac9a6-f4f8-438b-a60e-a2b4d3312102
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relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscovery2f459d84-881a-4d11-b81c-59ea5748f362
relation.isProjectOfPublication0f602f0c-00f1-4309-953a-58ef0de1ee6f
relation.isProjectOfPublication2488473c-6599-4873-9885-7638817cc0d5
relation.isProjectOfPublication.latestForDiscovery2488473c-6599-4873-9885-7638817cc0d5

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