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Advisor(s)
Abstract(s)
A simple and efficient protocol for the micropropagation of Drosera intermedia, using cultures initiated from in vitro
produced seedlings, is described. Shoot proliferation was significantly influenced by Murashige and Skoog (MS)
macronutrient concentration, showing higher multiplication rates for ¼ MS (the lowest concentration), but was not affected by the addition of 0.1 mg dm-3 kinetin. In all cases a multiplication percentage above 90 % was recorded. High rooting percentages (up to 100 %) were obtained in multiplication phase on ¼ MS medium without growth regulators. In average 15.8 plantlets per initial shoot was produced after 8 weeks of culture. All plantlets were successfully
acclimatized to ex vitro conditions, exhibiting normal development.
Description
Keywords
Carnivorous plant Droseraceae Kinetin
Citation
Grevenstuk T., Coelho N., Gonçalves S., Romano A. 2010. In vitro propagation of Drosera intermedia in a single step, Biologia Plantarum 54: 391-394