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Measurement of PTHrP, PTHR1, and CaSR expression levels in tissues of sea bream (Sparus aurata) using quantitative PCR

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dc.contributor.authorHang, X. M.
dc.contributor.authorPower, Deborah
dc.contributor.authorFlik, G.
dc.contributor.authorBalment, R.
dc.date.accessioned2014-10-23T15:10:30Z
dc.date.available2014-10-23T15:10:30Z
dc.date.issued2005
dc.description.abstractA quantitative PCR (Q-PCR) method has been established to measure the mRNA expression levels of parathyroid hormone–related protein (PTHrP), parathyroid hormone receptor type 1 (PTHR1), and calcium-sensing receptor (CaSR) in sea bream (Sparus aurata), using the housekeeping gene, - actin, as endogenous control. TaqMan primers and probes were designed using the Primer Express program, according to the published/unpublished sequences of the three target genes and -actin of sea bream. Different tissues including gill, kidney, duodenum, hindgut, rectum, liver, heart, brain, pituitary, skin, muscle, and gonad were removed and immediately snap-frozen from three juvenile sea bream (100–150 g) cultured in sea water. The mRNAs were extracted and reverse-transcribed into cDNAs, which were subsequently examined by the ABI 5700 system using an optimized Q-PCR method. Triplicate measures of each sample indicated consistency of the technique. However, the mRNA expression levels for each transcript in these tissues were variable between fish and also relatively low. Nevertheless, this methodology can be used in the future studies of factors that may alter gene expression in these tissues.por
dc.description.sponsorshipEU Project Grant No. Q5RS-2001-01465.por
dc.identifier.citationHang XM, Power D, Flik G, Balment RJ., "Measurement of PTHrP, PTHR1, and CaSR expression levels in tissues of sea bream (Sparus aurata) using quantitative PCR" in Ann N Y Acad Sci. 2005 Apr;1040:340-4.por
dc.identifier.doihttp://dx.doi.org/10.1196/annals.1327.056
dc.identifier.issn0077-8923
dc.identifier.otherAUT: DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/5433
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1196/annals.1327.056/abstractpor
dc.subjectQ-PCRpor
dc.subjectPTHrPpor
dc.subjectPTHRP1por
dc.subjectCaSRpor
dc.subjectSea breampor
dc.titleMeasurement of PTHrP, PTHR1, and CaSR expression levels in tissues of sea bream (Sparus aurata) using quantitative PCRpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage344por
oaire.citation.issue1
oaire.citation.startPage340por
oaire.citation.titleAnnals of The New York Academy of Sciencespor
oaire.citation.volume1040por
person.familyNamePower
person.givenNameDeborah Mary
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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