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Expression patterns of MLC isoforms during halibut (Hippoglossus hippoglossus L.) metamorphosis

2006Journal article Open access
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dc.contributor.authorSilva, Nadia
dc.contributor.authorBaptista, Vitor M.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-10-22T14:46:30Z
dc.date.available2014-10-22T14:46:30Z
dc.date.issued2006
dc.description.abstractAtlantic halibut is an important commercial fish in the countries of the North Atlantic and is emerging as a promising species for marine cold-water aquaculture. The axial musculature of the developing larvae is the largest and most rapidly growing tissue and during the transition from larval to adult muscle fibre types significant changes in fibre morphology and gene transcription occur. In fact the change in myotome height correlates well with different larval halibut stages. In the present study the spatial and temporal expression of myosin light chain 1 (MLC1), 2 (MLC2) and 3 (MLC3) was studied in metamorphosing halibut by in situ hybridization. As a first step to establishing a role for the thyroid axis in halibut muscle development whole body thyroid hormone (TH) concentrations were also determined. In first feeding larvae MLC1, MLC2A and MLC3 transcripts had a similar distribution and were confined to the muscle fibres of the germinal zones. In pre-metamorphic larvae transcripts were highly expressed throughout the epaxial and hypaxial musculature and expression levels reached a maximum in larvae starting metamorphosis, this change coincided with a significant increase in the concentration of thyroid hormones. By the time larvae reached the metamorphic climax, MLC1, MLC2A and MLC3 expression was still high throughout the musculature but expression was confined to fibres adjacent to the myosepts and to small cells scattered in the musculature, possibly satellite cells. MLC2A was also expressed in the red muscle fibres; no transition between larval and adult MLC isoforms was detected.por
dc.description.sponsorshipThis work was funded by European Union project CT 96-1442, ARRDE-Q5Rs-2002-01192 and Portuguese Ministry of Science and Technology – Pluriannual funding to CCMAR. We thank Heiddis Smáradóttir, (Fiskey, Iceland) for providing the animal samples.por
dc.identifier.citationNádia Silva, Vítor M. Baptista, Deborah Power, "Expression patterns of MLC isoforms during halibut (Hippoglossus hippoglossus L.) metamorphosis" in Arch. Tierz., Dummerstorf 49 (2006) Special Issue, 97-101.por
dc.identifier.otherAUT: DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/5412
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherLeibniz Institute for Farm Animal Biologypor
dc.subjectFlatfishpor
dc.subjectIn situ localisationpor
dc.subjectMuscle developmentpor
dc.subjectThyroid hormonespor
dc.titleExpression patterns of MLC isoforms during halibut (Hippoglossus hippoglossus L.) metamorphosispor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage101por
oaire.citation.startPage97por
oaire.citation.titleArchiv Tierzuchtpor
oaire.citation.volume49por
person.familyNameSilva
person.familyNamePower
person.givenNameNádia
person.givenNameDeborah Mary
person.identifier.ciencia-id5D1F-6F3A-A1C2
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-9881-4421
person.identifier.orcid0000-0003-1366-0246
person.identifier.scopus-author-id10244362100
person.identifier.scopus-author-id7101806760
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationa467188c-1363-4f77-a9a9-8297de10a01a
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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