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Glucose metabolism and gene expression in juvenile zebrafish (Danio rerio) challenged with a high carbohydrate diet: effects of an acute glucose stimulus during late embryonic life

2015-02Journal article Open access
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dc.contributor.authorRocha, Filipa
dc.contributor.authorDias, Jorge
dc.contributor.authorEngrola, S.
dc.contributor.authorGavaia, Paulo
dc.contributor.authorGeurden, Inge
dc.contributor.authorDinis, Maria Teresa
dc.contributor.authorPanserat, Stephane
dc.date.accessioned2018-12-07T14:53:01Z
dc.date.available2018-12-07T14:53:01Z
dc.date.issued2015-02
dc.description.abstractKnowledge on the role of early nutritional stimuli as triggers of metabolic pathways in fish is extremely scarce. The objective of the present study was to assess the long-term effects of glucose injection in the yolk (early stimulus) on carbohydrate metabolism and gene regulation in zebrafish juveniles challenged with a high-carbohydrate low-protein (HC) diet. Eggs were microinjected at 1 d post-fertilisation (dpf) with either glucose (2 M) or saline solutions. Up to 25 dpf, fish were fed a low-carbohydrate high-protein (LC) control diet, which was followed by a challenge with the HC diet. Survival and growth of 35 dpf juveniles were not affected by injection or the HC diet. Glucose stimulus induced some long-term metabolic changes in the juveniles, as shown by the altered expression of genes involved in glucose metabolism. On glycolysis, the expression levels of hexokinase 1 (HK1) and phosphofructokinase-6 (6PFK) were up-regulated in the visceral and muscle tissues, respectively, of juveniles exposed to the glucose stimulus, indicating a possible improvement in glucose oxidation. On gluconeogenesis, the inhibition of the expression levels of PEPCK in fish injected with glucose suggested lower production of hepatic glucose. Unexpectedly, fructose-1,6-bisphosphatase (FBP) expression was induced and 6PFK expression reduced by glucose stimulus, leaving the possibility of a specific regulation of the FBP-6PFK metabolic cycle. Glucose metabolism in juveniles was estimated using a [C-14]glucose tracer; fish previously exposed to the stimulus showed lower retention of [C-14]glucose in visceral tissue (but not in muscle tissue) and, accordingly, higher glucose catabolism, in comparison with the saline group. Globally, our data suggest that glucose stimulus at embryo stage has the potential to alter particular steps of glucose metabolism in zebrafish juveniles.
dc.description.sponsorshipFundacao para a Ciencia e Tecnologia (FCT, Portugal) [PTDC/CVT/102481/2008]; FCT (Portugal) [SFRH/BD/74921/2010, SFRH/BPD/49051/2008]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1017/S0007114514003869
dc.identifier.issn0007-1145
dc.identifier.issn1475-2662
dc.identifier.urihttp://hdl.handle.net/10400.1/11314
dc.language.isoeng
dc.peerreviewedyes
dc.publisherCambridge University Press
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectTrout Oncorhynchus-Mykiss
dc.subjectSole Solea-Senegalensis
dc.subjectCarp Cyprinus-Carpio
dc.subjectBream Sparus-Aurata
dc.subjectRainbow-Trout
dc.subjectAmino-acid
dc.subjectCommon Carp
dc.subjectNutritional regulation
dc.subjectGilthead seabream
dc.subjectTeleost fish
dc.titleGlucose metabolism and gene expression in juvenile zebrafish (Danio rerio) challenged with a high carbohydrate diet: effects of an acute glucose stimulus during late embryonic life
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCVT%2F102481%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F49051%2F2008/PT
oaire.citation.endPage413
oaire.citation.issue3
oaire.citation.startPage403
oaire.citation.titleBritish Journal of Nutrition
oaire.citation.volume113
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
person.familyNameRocha
person.familyNameEngrola
person.familyNameGavaia
person.familyNameDinis
person.givenNameFilipa
person.givenNameSofia
person.givenNamePaulo
person.givenNameMaria Teresa
person.identifier237846
person.identifier.ciencia-idA610-29E7-9B83
person.identifier.ciencia-idB619-FC16-D007
person.identifier.ciencia-id5414-4ACF-BF90
person.identifier.orcid0000-0002-7531-9302
person.identifier.orcid0000-0002-5244-5541
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0001-7813-1324
person.identifier.ridA-2485-2012
person.identifier.ridA-6470-2011
person.identifier.scopus-author-id8669620600
person.identifier.scopus-author-id6507104377
person.identifier.scopus-author-id7004150905
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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