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Thymus lotocephalus wild plants and in vitro cultures produce different profiles of phenolic compounds with antioxidant activity

2012Journal article Restricted
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dc.contributor.authorCosta, Patrícia
dc.contributor.authorGonçalves, Sandra
dc.contributor.authorValentão, Patrícia
dc.contributor.authorAndrade, Paula B.
dc.contributor.authorCoelho, Natacha
dc.contributor.authorRomano, Anabela
dc.date.accessioned2013-03-15T11:38:24Z
dc.date.available2013-03-15T11:38:24Z
dc.date.issued2012
dc.description.abstractWe compared the phenolic metabolites and antioxidant activities of Thymus lotocephalus G. López & R.Morales wild plants and in vitro cultures using different extraction solvents. HPLC–DAD analysis allowed the identification and quantification of phenolic (caffeic and rosmarinic) acids and flavones (luteolin and apigenin) in extracts from both sources. The in vitro cultures accumulated large amounts of rosmarinic acid. However, extracts from both sources were able to neutralise free radicals in different test systems(TEAC and ORAC assays), to form complexes with Fe2+ and to protect mouse brains against Fe2+-induced lipid peroxidation. The solvent significantly influenced the phenolic content and antioxidant activity of the extracts, water/ethanol being the most efficient for the extraction of antioxidant phytochemicals. We conclude that in vitro cultures of T. lotocephalus represent a promising alternative for the production of valuable natural antioxidants and an efficient tool for the in vitro biosynthesis of rosmarinic acid, therefore avoiding the need to exploit populations of wild plants.por
dc.description.sponsorshipP. Costa, S. Gonçalves and N. Coelho acknowledge grants from the Portuguese Science and Technology Foundation (FCT) (SFRH/BD/63505/2009, SFRH/BPD/31534/2006 and SFRH/BD/63501/2009, respectively). P. Valentão and P.B. Andrade are grateful to FCT for Grant No. PEst-C/EQB/LA0006/2011.por
dc.identifier.citationCosta P., Gonçalves S., Valentão P., Andrade P. B., Coelho N., Romano A 2012. Thymus lotocephalus wild plants and in vitro cultures produce different profiles of phenolic compounds with antioxidant activity, Food Chemistry 135: 1253–1260por
dc.identifier.issn0308-8146
dc.identifier.otherAUT: ARO01015;
dc.identifier.urihttp://hdl.handle.net/10400.1/2582
dc.language.isoengpor
dc.peerreviewedyespor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S030881461200903Xpor
dc.subjectThymus lotocephaluspor
dc.subjectWild plantspor
dc.subjectIn vitro culturespor
dc.subjectAntioxidant activitypor
dc.subjectPhenolic compoundspor
dc.titleThymus lotocephalus wild plants and in vitro cultures produce different profiles of phenolic compounds with antioxidant activitypor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1260por
oaire.citation.issue3por
oaire.citation.startPage1253por
oaire.citation.titleFood Chemistrypor
oaire.citation.volume135por
person.familyNameGonçalves
person.familyNameCoelho
person.familyNameRomano
person.givenNameSandra
person.givenNameNatacha
person.givenNameAnabela
person.identifier.ciencia-id9F19-E3AE-2C59
person.identifier.ciencia-idC518-BDB3-E127
person.identifier.ciencia-id8A18-7E9A-3B0E
person.identifier.orcid0000-0002-3038-4434
person.identifier.orcid0000-0003-4265-5622
person.identifier.orcid0000-0002-7204-7428
person.identifier.ridM-6988-2017
person.identifier.scopus-author-id8583003200
person.identifier.scopus-author-id56249997600
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationa8b335b2-45cc-4578-89b0-153273014280
relation.isAuthorOfPublication43b2d7ec-049a-412b-ad22-c9db64cc572c
relation.isAuthorOfPublicatione3343b60-17d3-4f5e-a2bf-06f26da69206
relation.isAuthorOfPublication.latestForDiscovery43b2d7ec-049a-412b-ad22-c9db64cc572c

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