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A fish scale in vitro bioassay to screen for endocrine disrupting compounds

2016Other Open access
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dc.contributor.authorPinto, Patricia IS
dc.contributor.authorEstêvão, Dulce
dc.contributor.authorSantos, Soraia
dc.contributor.authorAndrade, André
dc.contributor.authorPower, Deborah
dc.date.accessioned2020-01-28T10:52:06Z
dc.date.available2020-01-28T10:52:06Z
dc.date.issued2016
dc.description.abstractA wide range of natural and anthropogenic compounds are accumulating in the aquatic environment, many of which can interact with and disrupt the endocrine system. Estrogenic endocrine disruptors (EDCs) are a particular problem with impact on humans, ecosystems and wildlife and are particularly relevant in aquatic organisms like fish that may experience life-long exposures. The effects of EDCs in fish have mainly been assessed using reproductive endpoints and in vivo animal experiments. We propose that using other potential endpoints, such as the effect of estrogens on mineralized tissue, would allow development of a simple non invasive assay using scales. Fish scales are mineralized tissues that express both membrane and nuclear estrogen receptors, and are targets for natural estrogens and EDCs. The in vitro bioassay optimized in this work includes sampling of fish scales, incubation in culture media containing the tested compounds and measurement of enzymatic activities related to calcium turnover (TRAP, tartrate-resistant acid phosphatase and ALP, alkaline phosphatase). Several variables were optimized including culture media, compounds concentrations and incubation conditions (e.g. temperature, time), using both sea bass (Dicentrarchus labrax) and tilapia (Oreochromis mossambicus) scales. Significant effects of E2 and EDCs were detected, including both rapid (30 minutes) or slow (1day) changes in scale TRAP or ALP activities, but the responses were of low magnitude and varied with the individual, age, time of year, species and culture conditions. The in vitro fish scale assay is a promising non-invasive screening tool for E2 and EDCs effects, complying with the 3Rs of animal welfare. However, current technical limitations are its limited sensitivity for some parameters eg. TRAP/ALP activity and alternative, sensitive, robust and easy to measure endpoints are under investigation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPinto, P. I. S., Estêvão, M. D., Santos, S., Andrade, A., Power, D. M. (2016). A fish scale in vitro bioassay to screen for endocrine disrupting compounds using fish scales. 30th New European Society for Comparative Physiology and Biochemistry Congress (ESCPB 2016), Barcelona, 4-7/9/2016.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.1/13429
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationE2SCALE: Scaling-down estrogenic responses in fish
dc.relationStrategic Project - LA 15 - 2011-2012
dc.titleA fish scale in vitro bioassay to screen for endocrine disrupting compoundspt_PT
dc.typeother
dspace.entity.typePublication
oaire.awardTitleE2SCALE: Scaling-down estrogenic responses in fish
oaire.awardTitleStrategic Project - LA 15 - 2011-2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F84033%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAAG-GLO%2F4003%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FMAR%2FLA0015%2F2011/PT
oaire.citation.conferencePlaceBarcelonapt_PT
oaire.citation.title30th New European Society for Comparative Physiology and Biochemistry Congress (ESCPB 2016)pt_PT
oaire.fundingStreamSFRH
oaire.fundingStream3599-PPCDT
oaire.fundingStream6820 - DCRRNI ID
person.familyNamePinto
person.familyNameEstêvão
person.familyNameSantos
person.familyNameAlçada Baptista Rebelo de Andrade
person.familyNamePower
person.givenNamePatricia IS
person.givenNameMaria Dulce da Mota Antunes de Oliveira
person.givenNameSoraia
person.givenNameAndré
person.givenNameDeborah Mary
person.identifier643457
person.identifierI-4695-2014
person.identifier.ciencia-idE51D-5CCB-B1C6
person.identifier.ciencia-id881B-53E8-0DB1
person.identifier.ciencia-idEF18-F0AF-D388
person.identifier.ciencia-idF513-56ED-7088
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-7854-3898
person.identifier.orcid0000-0002-7151-8363
person.identifier.orcid0000-0001-5006-2021
person.identifier.orcid0000-0002-5900-8782
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-3817-2013
person.identifier.ridC-1328-2018
person.identifier.scopus-author-id10240774300
person.identifier.scopus-author-id8381590400
person.identifier.scopus-author-id7101806760
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeotherpt_PT
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