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Differential protein expression in mussels Mytilus galloprovincialis exposed to nano and ionic Ag

2013Journal article Restricted access
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dc.contributor.authorGomes, Tânia
dc.contributor.authorG. Pereira, Catarina
dc.contributor.authorCardoso, Cátia
dc.contributor.authorBebianno, Maria João
dc.date.accessioned2020-04-27T10:08:20Z
dc.date.available2020-04-27T10:08:20Z
dc.date.issued2013
dc.description.abstractAg NPs are one of the most commonly used NPs in nanotechnology whose environmental impacts are to date unknown and the information about bioavailability, mechanisms of biological uptake and toxic implications in organisms is scarce. So, the main objective of this study was to investigate differences in protein expression profiles in gills and digestive gland of mussels Mytilus galloprovincialis exposed to Ag NPs and Ag(+) (10 μg L(-1)) for a period of 15 days. Protein expression profiles of exposed gills and digestive glands were compared to those of control mussels using two-dimensional electrophoresis to discriminate differentially expressed proteins. Different patterns of protein expression were obtained for exposed mussels, dependent not only on the different redox requirements of each tissue but also to the Ag form used. Unique sets of differentially expressed proteins were affected by each silver form in addition to proteins that were affected by both Ag NPs and Ag(+). Fifteen of these proteins were subsequently identified by MALDI-TOF-TOF and database search. Ag NPs affected similar cellular pathways as Ag(+), with common response mechanisms in cytoskeleton and cell structure (catchin, myosin heavy chain), stress response (heat shock protein 70), oxidative stress (glutathione s-transferase), transcriptional regulation (nuclear receptor subfamily 1G), adhesion and mobility (precollagen-P) and energy metabolism (ATP synthase F0 subunit 6 and NADH dehydrogenase subunit 2). Exposure to Ag NPs altered the expression of two proteins associated with stress response (major vault protein and ras partial) and one protein involved in cytoskeleton and cell structure (paramyosin), while exposure to Ag(+) had a strong influence in one protein related to stress response (putative c1q domain containing protein) and two proteins involved in cytoskeleton and cell structure (actin and α-tubulin). Protein identification showed that Ag NPs toxicity is mediated by oxidative stress-induced cell signalling cascades (including mitochondria and nucleus) that can lead to cell death. This toxicity represents the cumulative effect of Ag(+) released from the particles and other properties as particle size and surface reactivity. This study helped to unravel the molecular mechanisms that can be associated with Ag NPs toxicity; nevertheless, some additional studies are required to investigate the exact interaction between these NPs and cellular components.pt_PT
dc.description.sponsorshipFP7- PEOPLE-2009-IRSESpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.aquatox.2013.03.021pt_PT
dc.identifier.issn0166-445X
dc.identifier.urihttp://hdl.handle.net/10400.1/13783
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationEFEITOS DA EXPOSIÇÃO A NANOPARTÍCULAS NA ESPÉCIE MYTILUS GALLOPROVINCIALIS
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectElectrophoresispt_PT
dc.subjectEnvironmental pollutantspt_PT
dc.subjectGene expression regulationpt_PT
dc.subjectGillspt_PT
dc.subjectMass spectrometrypt_PT
dc.subjectMetal nanoparticlespt_PT
dc.subjectMytiluspt_PT
dc.subjectProteomicspt_PT
dc.subjectSilverpt_PT
dc.subjectSpectrophotometrypt_PT
dc.subjectAtomicpt_PT
dc.subjectGelpt_PT
dc.subjectTwo-dimensionalpt_PT
dc.titleDifferential protein expression in mussels Mytilus galloprovincialis exposed to nano and ionic Agpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEFEITOS DA EXPOSIÇÃO A NANOPARTÍCULAS NA ESPÉCIE MYTILUS GALLOPROVINCIALIS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F41605%2F2007/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PTDC%2FAAC-AMB%2F121650%2F2010/PT
oaire.citation.endPage90pt_PT
oaire.citation.startPage79pt_PT
oaire.citation.titleAquatic Toxicologypt_PT
oaire.citation.volume136-137pt_PT
oaire.fundingStreamCOMPETE
person.familyNameGomes
person.familyNameGuerreiro Pereira
person.familyNameCardoso
person.familyNameBebianno
person.givenNameTânia
person.givenNameCatarina Alexandra
person.givenNameCátia
person.givenNameMaria
person.identifierNmxO5SIAAAAJ
person.identifier.ciencia-id4512-3435-689C
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0001-6179-4543
person.identifier.orcid0000-0002-1131-8773
person.identifier.orcid0000-0003-3157-9898
person.identifier.orcid0000-0003-1492-8566
person.identifier.ridI-9235-2014
person.identifier.scopus-author-id7004152715
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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