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Chitosan/sulfated locust bean gum nanoparticles: In vitro and in vivo evaluation towards an application in oral immunization

2017-03Journal article Open access
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dc.contributor.authorBraz, L.
dc.contributor.authorGrenha, Ana
dc.contributor.authorFerreira, Domingos
dc.contributor.authorRosa Da Costa, Ana
dc.contributor.authorGamazo, Carlos
dc.contributor.authorSarmento, Bruno
dc.date.accessioned2019-11-20T15:07:47Z
dc.date.available2019-11-20T15:07:47Z
dc.date.issued2017-03
dc.description.abstractThis work proposes the design of nanoparticles based on locus bean gum (LBG) and chitosan to be used as oral immunoadjuvant for vaccination purposes. LBG-based nanoparticles were prepared by mild polyelectrolyte complexation between chitosan (CS) and a synthesized LBG sulfate derivative (LBGS). Morphological characterization suggested that nanoparticles present a solid and compact structure with spherical-like shape. Sizes around 180-200 nm and a positive surface charge between +9 mV and +14 mV were obtained. CS/LBGS nanoparticles did not affect cell viability of Caco-2 cells after 3 h and 24h of exposure when tested at concentrations up to 1.0 mg/mL. Two model antigens (a particulate acellular extract HE of Salmonella enterica serovar Enteritidis, and ovalbumin as soluble antigen) were associated to CS/LBGS nanoparticles with efficiencies around 26% for ovalbumin and 32% for HE, which resulted in loading capacities up to 12%. The process did not affect the antigenicity of the associated antigens. BALB/c mice were orally immunized with ovalbumin-loaded nanoparticles (100 mu g), and results indicate an adjuvant effect of the CS/LBGS nanoparticles, eliciting a balanced Th1/Th2 immune response. Thus, CS/LBGS nanoparticles are promising as antigen mucosal delivery strategy, with particular interest for oral administration. (C) 2017 Elsevier B.V. All rights reserved.
dc.description.sponsorshipNational Portuguese funding through FCT- Fundacao para a Ciencia e a Tecnologia, project [PTDC/SAU-FCF/100291/2008, PEst-OE/EQB/LA0023/2011]
dc.description.sponsorshipFEDER - Fundo Europeu de Desenvolvimento Regional
dc.description.sponsorshipPortuguese funds through FCT - Fundacao para a Ciencia e a Tecnologia/Ministerio da Ciencia
dc.identifier.doi10.1016/j.ijbiomac.2016.12.076
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.urihttp://hdl.handle.net/10400.1/13217
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.subjectPolyelectrolyte complex nanoparticles
dc.subjectLoaded chitosan nanoparticles
dc.subjectVaccine delivery
dc.subjectImmune-response
dc.subjectSalmonella-enteritidis
dc.subjectHepatitis-B
dc.subjectChitosan/Carrageenan nanoparticles
dc.subjectImmunoadjuvant properties
dc.subjectBioadhesive capacity
dc.subjectProtein delivery
dc.titleChitosan/sulfated locust bean gum nanoparticles: In vitro and in vivo evaluation towards an application in oral immunization
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-FCF%2F100291%2F2008/PT
oaire.citation.endPage797
oaire.citation.startPage786
oaire.citation.titleInternational Journal of Biological Macromolecules
oaire.citation.volume96
oaire.fundingStream3599-PPCDT
person.familyNameBraz
person.familyNameGrenha
person.familyNameRosa da Costa
person.givenNameLuis
person.givenNameAna
person.givenNameAna M
person.identifier305029
person.identifier.ciencia-idBA15-0A43-361F
person.identifier.ciencia-id091C-0D58-7225
person.identifier.ciencia-idA418-A85E-5DB1
person.identifier.orcid0000-0002-1500-1976
person.identifier.orcid0000-0002-2136-1396
person.identifier.orcid0000-0003-0225-9537
person.identifier.ridE-1901-2012
person.identifier.ridH-1392-2017
person.identifier.ridE-2165-2012
person.identifier.scopus-author-id55996112700
person.identifier.scopus-author-id8607930100
person.identifier.scopus-author-id53986075100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a CiĂȘncia e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
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