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Molecular and cellular effects of temperature in oysters Crassostrea brasiliana exposed to phenanthrene

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dc.contributor.authorLima, Daína
dc.contributor.authorZacchi, Flávia Lucena
dc.contributor.authorMattos, Jacó Joaquim
dc.contributor.authorFlores-Nunes, Fabrício
dc.contributor.authorGomes, Carlos Henrique Araújo de Miranda
dc.contributor.authorde Mello, Álvaro Cavaler Pessoa
dc.contributor.authorSiebert, Marília Nardelli
dc.contributor.authorPiazza, Clei Endrigo
dc.contributor.authorTaniguchi, Satie
dc.contributor.authorSasaki, Silvio Tarou
dc.contributor.authorBícego, Márcia Caruso
dc.contributor.authorBebianno, Maria João
dc.contributor.authorde Almeida, Eduardo Alves
dc.contributor.authorBainy, Afonso Celso Dias
dc.date.accessioned2020-04-07T10:35:44Z
dc.date.available2020-04-07T10:35:44Z
dc.date.issued2018
dc.description.abstractExposure of aquatic organisms to polycyclic aromatic hydrocarbons (PAH), such as phenanthrene (PHE), may increase the production of reactive oxygen species (ROS) and cause changes in the biotransformation systems. In addition, changes in water temperature can cause adverse effects in the organisms. Estuarine species, like the oyster Crassostrea brasiliana, can adapt and tolerate temperature variation. To evaluate the influence of temperature on biological responses of C. brasiliana exposed to PHE, oysters were maintained at three temperatures (18, 24 and 32 °C) for 15 days and co-exposed afterwards to 100 μg.L-1 of PHE for 24 and 96 h. Levels of PHE in the water and oyster tissues were determined, respectively after 24 and 96 h. In addition, thermal stress, biotransformation and oxidative stress-related genes were analyzed in oyster gills, together with the activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferases (GST) and levels of lipid peroxidation. Oyster accumulated significant levels of PHE. HSP70-like transcripts were affected by PHE exposure only at 32 °C. Transcript levels of cytochrome P450 isoforms (CYP2-like2 and CYP2AU1) were down-regulated in oysters exposed to PHE for 24 h at 32 °C. GSTΩ-like transcript levels were also down-regulated in the PHE-exposed group at 32 °C. After 96 h, CYP2-like2 transcripts were higher in the PHE exposed groups at 32 °C. Oysters kept at 18 °C showed higher levels of SOD-like transcripts, together with higher GST, GPx and G6PDH activities, associated to lower levels of lipoperoxidation. In general the biological responses evaluated were more affected by temperature, than by co-exposure to PHE.pt_PT
dc.description.sponsorshipCNPq: 573949/2008-5, 483028/2012-6, 2013/07937-8, 406104/2013-1, CNPq 307467/2013-9pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doihttps://doi.org/10.1016/j.chemosphere.2018.06.094pt_PT
dc.identifier.issn0045-6535
dc.identifier.urihttp://hdl.handle.net/10400.1/13698
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S004565351831169X?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectTemperaturept_PT
dc.subjectCrassostrea brasilianapt_PT
dc.subjectPhenanthrenept_PT
dc.subjectBiotransformationpt_PT
dc.subjectOxidative stresspt_PT
dc.titleMolecular and cellular effects of temperature in oysters Crassostrea brasiliana exposed to phenanthrenept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMAR%2F00350%2F2013/PT
oaire.citation.endPage318pt_PT
oaire.citation.startPage307pt_PT
oaire.citation.titleChemospherept_PT
oaire.citation.volume209pt_PT
oaire.fundingStream5876
person.familyNameBebianno
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isProjectOfPublication3c9090f1-c8fc-4990-8cd8-d61d72eb13f7
relation.isProjectOfPublication.latestForDiscovery3c9090f1-c8fc-4990-8cd8-d61d72eb13f7

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