Cloning and characterisation of a fish aldolase B gene

Llewellyn, Lynda; Ramsurn, Vimi P.; Sweeney, Glen E.; Wigham, Trevor; Santos, Cecilia; Power, Deborah

http://hdl.handle.net/10400.1/5385

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Abstract(s)

A full length cDNA clone representing an aldolase mRNA was isolated from a sea bream (Sparus aurutu) liver cDNA library. Sequencing of this clone revealed it to encode a 364 amino acid protein with 74% amino acid identity to human aldolase B and slightly lower similarity to human aldolase A and C. In view of the sequence data and of Northern blot analysis showing strong expression of a 1.6 kb transcript in liver it was concluded that the cloned gene represents aldolase B. This clone represents the first aldolase gene to be sequenced from any fish species thus providing new data on the evolution of the vertebrate aldolase gene family.

Description

E.C. (contract No. AIR2-CT93-1483).

Keywords

Aldolase Fish Sea bream Sparus aurta

URI

http://hdl.handle.net/10400.1/5385

Citation

Llewellyn L1, Ramsurn VP, Sweeney GE, Wigham T, Santos CR, Power DM. Cloning and characterisation of a fish aldolase B gene. Biochim Biophys Acta. 1995 Jul 25;1263(1):75-8.